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|Antigen||CD4 Molecule (CD4) Antibodies|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunoprecipitation (IP)
|2 references available|
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Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120326) Images
|Purification||Affinity Chromatography on Protein G.|
|Immunogen||293T cells expressing Pekin duck CD4. Spleen cells from immunised Balb/c mice were fused with cells of the SP2/0 mouse myeloma cell line.|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120326) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4 is shown to be expressed by thymocytes, splenocytes and peripheral lymphoid cells. Since the majority of avian immune studies have been carried out on chickens, relatively little is known about the immune system of ducks, though there is a resemblance between the main lymphoid organs, the spleen, thymus and bursa of Fabricius. At the cellular level, studies have shown that like mammalian T cells, duck lymphocytes are responsive to phytohaemagglutinin (PHA), and all cells reacting with clone Du CD4-2 have been identified as CD3+ T cells. (1)Synonyms: T-Cell Surface Antigen T4/Leu-3, T-cell Surface Glycoprotein CD4, T4/Leu-3|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
|Pathways||TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN120326) Images back to top
Flow Cytometry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN120326) Images back to top
|Buffer||PBS containing 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ProductDetails: References for anti-CD4 antibody (ABIN120326)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Kothlow, Mannes, Schaerer, Rebeski, Kaspers, Schultz: "Characterization of duck leucocytes by monoclonal antibodies." in: Developmental and comparative immunology, Vol. 29, Issue 8, pp. 733-48, 2005
Higgins, Teoh: "Duck lymphocytes. II. Culture conditions for optimum transformation response to phytohaemagglutinin." in: Journal of immunological methods, Vol. 106, Issue 1, pp. 135-45, 1988