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anti-Human CD4 antibody for Immunocytochemistry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Human
1197
694
240
69
52
45
25
23
22
21
19
15
14
12
9
8
7
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Mouse
1361
667
276
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to PE-Cy5
284
239
176
114
44
42
42
39
38
38
36
32
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
7
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)
1808
436
399
356
309
300
178
136
99
70
33
28
28
27
22
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN2144349
$ 275.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2144347 ICC FACS IF PE-Cy5 Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2145044 ICC FACS IF Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2144463 ICC FACS IF FITC Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2145043 ICC FACS IF Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2144757 ICC FACS IF PE Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2144465 ICC FACS IF FITC Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2144754 ICC FACS IF PE Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2144213 ICC FACS IF APC Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2144211 ICC FACS IF APC Mouse IgG2a Log in to see 4AEDU-2 2
0.0009962841 ABIN2662087 Func ICC FACS IF ICFC IP WB Pacific Blue Mouse IgG2b kappa Log in to see OKT4 8
0.0009962841 ABIN2662088 Func ICC FACS IF ICFC IP WB Pacific Blue Mouse IgG2b kappa Log in to see OKT4 8
0.0009962841 ABIN2506709 ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN2883598 ICC IHC ELISA Rabbit IgG pSer433 Log in to see Polyclonal
0.0009962841 ABIN1077935 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 26-390 Log in to see Polyclonal
0.0009962841 ABIN1100597 ICC IHC (fro) FACS Mouse IgG2a Log in to see RIV7
0.0009962841 ABIN2567616 Func ICC FACS IF IP WB Mouse IgG2b kappa Log in to see OKT4
0.0009962841 ABIN2506618 ICC IF FITC Rabbit IgG Log in to see Polyclonal
0.0009962841 ABIN4292751 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0009962841 ABIN4292752 ELISA ICC IF IHC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0009962841 ABIN4292746 ICC IF IHC IHC (p) SimWes WB Rabbit AA 400-458, C-Term Log in to see Polyclonal

Top referenced anti-CD4 antibody for Immunocytochemistry

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Application / Reactivity Human
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Biochemical Assay (BCA) 6 Antibodies
Blocking Antibody (Inhibition) 1 Antibodies
Blocking Reagent (BR) 9 Antibodies
Complement Mediated Cell Depletion (CMCD) 2 Antibodies
Depletion (Dep) 4 Antibodies
ELISA 249 Antibodies
ELISA (Capture) 1 Antibodies
ELISA (Detection) 1 Antibodies
ELISpot (Detection) (ELISPOT (Detection)) 1 Antibodies
Enzyme Immunoassay (EIA) 6 Antibodies
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Functional Studies (Func) 32 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 43 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Human
(1197), (694), (240), (69), (52), (45), (25), (23), (22), (21), (19), (15), (14), (12), (9), (8), (7), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Mouse
(1361), (667), (276), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to PE-Cy5
(284), (239), (176), (114), (44), (42), (42), (39), (38), (38), (36), (32), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (7), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)
(1808), (436), (399), (356), (309), (300), (178), (136), (99), (70), (33), (28), (28), (27), (22), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN2144349) Images
Characteristics CD4, also known as T4, is a 55 kD single chain transmembrane glycoprotein and belongs to immunoglobulin superfamily.
Clone 4AEDU-2
Isotype IgG2a

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN2144349) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is found on most thymocytes, a subset of T cells and at low level on monocytes/macrophages.CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction.
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN2144349) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Assay Procedure
  • Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
  • Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
  • Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
  • Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
  • Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
  • [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
  • [Cell fixation: 2 % formaldehyde solution]
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN2144349) Images back to top
Format Liquid
Buffer Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C

References for anti-CD4 Antibody (ABIN2144349)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Clevers, Alarcon, Wileman, Terhorst: "The T cell receptor/CD3 complex: a dynamic protein ensemble." in: Annual review of immunology, Vol. 6, pp. 629-62, 1988

Ledbetter, Evans, Lipinski, Cunningham-Rundles, Good, Herzenberg: "Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and cytotoxic/suppressor subpopulations in mouse and man." in: The Journal of experimental medicine, Vol. 153, Issue 2, pp. 310-23, 1981

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN2144349) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule)  (PE-Cy5) (ABIN2144349) anti-CD4 Molecule (CD4) antibody (PE-Cy5)