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|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD4 antibody is conjugated to Biotin Alternatives|
Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP)
|4 references available|
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Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN234619) Images
|Specificity||Recognizes a determinant designated L3T4 present on mouse T-cell surface with an apparent molecular mass of 52 kDa. The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.|
|Characteristics||MAb to Mouse CD4 (L3T4), Monoclonal antibody to Mouse CD4 (L3T4) Biotin conjugated|
|Purification||Protein G Chromatography. Fluorescein which has a maximum absorbance at 492 nm and an emission maximum at 518 nm has been covalently attached to Anti-MAP-2 and purified to assure optimal fluorochrome/protein (F/P) molar ratios. Cell culture|
|Immunogen||Lyt-2-negative T-cell clone 243/2.5|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN234619) Images back to top
|Alternative Name||CD4 (L3T4) (CD4 Antibody Abstract)|
|Pathways||TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN234619) Images back to top
|Application Notes||Useful for studies of T-helper cell functions including blocking of T-helper cell activation. The anti CD4 antibody can be used for FACS analysis, immunofluorescence, depletion and immunoprecipitation. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric application. Each laboratory should determine their own working dilution for individual use. Other applications have not been tested but use in such assays should not necessarily be excluded.|
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN234619) Images back to top
|Buffer||0.01 M PBS, pH 7.4 containing 1 % BSA, 0.01 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
DO NOT FREEZE!
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
|Storage Comment||Store at 2-8 °C.|
References for anti-CD4 antibody (ABIN234619)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Wilde, Just, Aktories: "Structure-function analysis of the Rho-ADP-ribosylating exoenzyme C3stau2 from Staphylococcus aureus." in: Biochemistry, Vol. 41, Issue 5, pp. 1539-44, 2002
Benjamin, Cobbold, Clark, Waldmann: "Tolerance to rat monoclonal antibodies. Implications for serotherapy." in: The Journal of experimental medicine, Vol. 163, Issue 6, pp. 1539-52, 1986
Waldor, Sriram, Hardy, Herzenberg, Herzenberg, Lanier, Lim, Steinman: "Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker." in: Science (New York, N.Y.), Vol. 227, Issue 4685, pp. 415-7, 1985
Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983