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anti-Mouse (Murine) CD4 antibody for Indirect Immunofluorescence Assay

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Mouse (Murine)
1198
694
241
72
53
45
25
23
22
21
19
15
15
15
10
9
8
7
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Rat
1372
669
268
4
2
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to Biotin
286
239
177
113
44
43
42
39
39
38
35
33
26
24
22
22
21
20
19
19
19
19
19
16
15
13
9
8
8
8
7
6
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP)
1815
435
400
366
306
303
181
141
104
70
33
29
28
27
23
18
14
11
10
9
7
6
6
6
5
5
4
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN234619
$ 453.20
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008630328 ABIN2772075 FACS IFA IP Biotin Rat IgG2b Log in to see

Top referenced anti-CD4 antibody for Indirect Immunofluorescence Assay

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 49 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 564 Antibodies
Functional Studies (Func) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 41 Antibodies
Immunofluorescence (IF) 70 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 105 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 7 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 94 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 19 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 5 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1198), (694), (241), (72), (53), (45), (25), (23), (22), (21), (19), (15), (15), (15), (10), (9), (8), (7), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rat
(1372), (669), (268), (4), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to Biotin
(286), (239), (177), (113), (44), (43), (42), (39), (39), (38), (35), (33), (26), (24), (22), (22), (21), (20), (19), (19), (19), (19), (19), (16), (15), (13), (9), (8), (8), (8), (7), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP)
(1815), (435), (400), (366), (306), (303), (181), (141), (104), (70), (33), (29), (28), (27), (23), (18), (14), (11), (10), (9), (7), (6), (6), (6), (5), (5), (4), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN234619) Images
Specificity Recognizes a determinant designated L3T4 present on mouse T-cell surface with an apparent molecular mass of 52 kDa. The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Characteristics MAb to Mouse CD4 (L3T4), Monoclonal antibody to Mouse CD4 (L3T4) Biotin conjugated
Purification Protein G Chromatography. Fluorescein which has a maximum absorbance at 492 nm and an emission maximum at 518 nm has been covalently attached to Anti-MAP-2 and purified to assure optimal fluorochrome/protein (F/P) molar ratios. Cell culture
Immunogen Lyt-2-negative T-cell clone 243/2.5
Clone GK1-5
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN234619) Images back to top
Antigen
Alternative Name CD4 (L3T4) (CD4 Antibody Abstract)
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN234619) Images back to top
Application Notes Useful for studies of T-helper cell functions including blocking of T-helper cell activation. The anti CD4 antibody can be used for FACS analysis, immunofluorescence, depletion and immunoprecipitation. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric application. Each laboratory should determine their own working dilution for individual use. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN234619) Images back to top
Format Liquid
Buffer 0.01 M PBS, pH 7.4 containing 1 % BSA, 0.01 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage 4 °C
Storage Comment Store at 2-8 °C.

References for anti-CD4 Antibody (ABIN234619)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Wilde, Just, Aktories: "Structure-function analysis of the Rho-ADP-ribosylating exoenzyme C3stau2 from Staphylococcus aureus." in: Biochemistry, Vol. 41, Issue 5, pp. 1539-44, 2002

Benjamin, Cobbold, Clark, Waldmann: "Tolerance to rat monoclonal antibodies. Implications for serotherapy." in: The Journal of experimental medicine, Vol. 163, Issue 6, pp. 1539-52, 1986

Waldor, Sriram, Hardy, Herzenberg, Herzenberg, Lanier, Lim, Steinman: "Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker." in: Science (New York, N.Y.), Vol. 227, Issue 4685, pp. 415-7, 1985

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983