anti-Mouse (Murine) CD4 antibody for Indirect Immunofluorescence Assay

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1251
655
235
78
68
41
36
28
27
26
22
21
19
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rat
1422
610
283
16
6
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is conjugated to Biotin
264
232
169
108
54
54
54
48
36
34
34
33
30
26
22
21
21
20
19
19
18
18
18
18
16
15
15
13
13
9
9
8
8
6
5
5
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP)
1822
495
453
353
323
310
161
156
98
78
41
39
38
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Options
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Catalog No. ABIN234619
$ 484.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2772075 FACS IFA IP Biotin Rat IgG2b Log in to see

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1251), (655), (235), (78), (68), (41), (36), (28), (27), (26), (22), (21), (19), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1422), (610), (283), (16), (6), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is conjugated to Biotin
(264), (232), (169), (108), (54), (54), (54), (48), (36), (34), (34), (33), (30), (26), (22), (21), (21), (20), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (9), (9), (8), (8), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Indirect Immunofluorescence Assay (IFA), Immunoprecipitation (IP)
(1822), (495), (453), (353), (323), (310), (161), (156), (98), (78), (41), (39), (38), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 antibody (ABIN234619) Images
Specificity Recognizes a determinant designated L3T4 present on mouse T-cell surface with an apparent molecular mass of 52 kDa. The L3T4 antigen is expressed by the helper/inducer subset of mouse T-cells. The antigen is present on approximately 80% of thymocytes, 20% of spleen cells and 60% of lymph node cells. The expression of L3T4 correlates with class II MHC antigen reactivity on cloned T-cell lines.
Characteristics MAb to Mouse CD4 (L3T4), Monoclonal antibody to Mouse CD4 (L3T4) Biotin conjugated
Purification Protein G Chromatography. Fluorescein which has a maximum absorbance at 492 nm and an emission maximum at 518 nm has been covalently attached to Anti-MAP-2 and purified to assure optimal fluorochrome/protein (F/P) molar ratios. Cell culture
Immunogen Lyt-2-negative T-cell clone 243/2.5
Clone GK1-5
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 antibody (ABIN234619) Images back to top
Antigen
Alternative Name CD4 (L3T4) (CD4 Antibody Abstract)
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 antibody (ABIN234619) Images back to top
Application Notes Useful for studies of T-helper cell functions including blocking of T-helper cell activation. The anti CD4 antibody can be used for FACS analysis, immunofluorescence, depletion and immunoprecipitation. We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric application. Each laboratory should determine their own working dilution for individual use. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 antibody (ABIN234619) Images back to top
Format Liquid
Buffer 0.01 M PBS, pH 7.4 containing 1 % BSA, 0.01 % Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice DO NOT FREEZE!
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use.
Storage 4 °C
Storage Comment Store at 2-8 °C.

References for anti-CD4 antibody (ABIN234619)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Wilde, Just, Aktories: "Structure-function analysis of the Rho-ADP-ribosylating exoenzyme C3stau2 from Staphylococcus aureus." in: Biochemistry, Vol. 41, Issue 5, pp. 1539-44, 2002

Benjamin, Cobbold, Clark, Waldmann: "Tolerance to rat monoclonal antibodies. Implications for serotherapy." in: The Journal of experimental medicine, Vol. 163, Issue 6, pp. 1539-52, 1986

Waldor, Sriram, Hardy, Herzenberg, Herzenberg, Lanier, Lim, Steinman: "Reversal of experimental allergic encephalomyelitis with monoclonal antibody to a T-cell subset marker." in: Science (New York, N.Y.), Vol. 227, Issue 4685, pp. 415-7, 1985

Dialynas, Wilde, Marrack, Pierres, Wall, Havran, Otten, Loken, Pierres, Kappler et al.: "Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with ..." in: Immunological reviews, Vol. 74, pp. 29-56, 1983