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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||3 Antibodies|
|Blocking Reagent (BR)||15 Antibodies|
|Cytotoxicity Test (CyTox)||7 Antibodies|
|Depletion (Dep)||2 Antibodies|
|ELISA (Capture)||2 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||469 Antibodies|
|Functional Studies (Func)||29 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||35 Antibodies|
|Immunofluorescence (IF)||64 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||100 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||10 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||56 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||5 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Blocking Reagent (BR), Cytotoxicity Test (CyTox), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP)
|14 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN1176846) Images
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Sterility||0.2 μm filtered|
|Endotoxin Level||Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.|
|Immunogen||A.TH mouse CTL clone A15.1.17|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN1176846) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
The H129.19 antibody reacts with the CD4 (L3T4) differentiation antigen expressed on thymocytes, a subpopulation of mature T lymphocytes (i.e., MHC class II-restricted T cells, including most T helper cells), and a subset of NK-T cells of all mouse strains tested. CD4 has also been detected on pluirpotent hematopoietic stem cells, bone marrow myeloid precursors, intrathymic lymphoid precursors, and a subset of splenic dendritic cells. CD4 is expressed on the plasma membrane of mouse egg cells and is involved in adhesion of the egg to MHC class II-bearing sperm. CD4 is an antigen coreceptor on the T-cell surface which interacts with MHC class II molecules on antigen-presenting cells. It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine lck. H129.19 mAb blocks binding of the anti-mouse CD4 mAbs Gk1.5 (Cat. No. 557307/553729) and RM4-5 (Cat. No. 553046/553047), but not RM4-4 (Cat. No. 553055) antibody. mAb H129.19 inhibits various responses of T helper cells to antigenic or mitogenic stimuli. This antibody is routinely tested by flow cytometric analysis.
|Research Area||Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN1176846) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN1176846) Images back to top
|Buffer||No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.|
|Storage Comment||Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.|
References for anti-CD4 Antibody (ABIN1176846)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
|Product cited in:||
Martín, del Hoyo, Anjuère et al.: "Concept of lymphoid versus myeloid dendritic cell lineages revisited: both CD8alpha(-) and CD8alpha(+) dendritic cells are generated from CD4(low) lymphoid-committed precursors." in: Blood, Vol. 96, Issue 7, pp. 2511-9, 2000 (PubMed).
Guo, Watanabe, Mori et al.: "Molecular structure and function of CD4 on murine egg plasma membrane." in: Zygote (Cambridge, England), Vol. 3, Issue 1, pp. 65-73, 1995 (PubMed).
Bendelac: "Mouse NK1+ T cells." in: Current opinion in immunology, Vol. 7, Issue 3, pp. 367-74, 1995 (PubMed).
Godfrey, Kennedy, Mombaerts et al.: "Onset of TCR-beta gene rearrangement and role of TCR-beta expression during CD3-CD4-CD8- thymocyte differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 10, pp. 4783-92, 1994 (PubMed).
Pierres, Naquet, Van Agthoven et al.: "A rat anti-mouse T4 monoclonal antibody (H129.19) inhibits the proliferation of Ia-reactive T cell clones and delineates two phenotypically distinct (T4+, Lyt-2,3-, and T4-, Lyt-2,3+) subsets among ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 132, Issue 6, pp. 2775-82, 1984 (PubMed).
Pont, Régnier-Vigouroux, Marchetto et al.: "Accessory molecules and T cell activation. II. Antibody binding to L3T4a inhibits Ia-independent mouse T cell proliferation." in: European journal of immunology, Vol. 17, Issue 3, pp. 429-32, 1987 (PubMed).
Naquet, Malissen, Bekkhoucha et al.: "L3T4 but not LFA-1 participates in antigen presentation by Ak-positive L-cell transformants." in: Immunogenetics, Vol. 22, Issue 3, pp. 247-56, 1985 (PubMed).
Ghobrial, Boublik, Winn et al.: "In vivo use of monoclonal antibodies against murine T cell antigens." in: Clinical immunology and immunopathology, Vol. 52, Issue 3, pp. 486-506, 1989 (PubMed).
Frederickson, Basch: "L3T4 antigen expression by hemopoietic precursor cells." in: The Journal of experimental medicine, Vol. 169, Issue 4, pp. 1473-8, 1989 (PubMed).
Bierer, Sleckman, Ratnofsky et al.: "The biologic roles of CD2, CD4, and CD8 in T-cell activation." in: Annual review of immunology, Vol. 7, pp. 579-99, 1989 (PubMed).
Wu, Scollay, Egerton et al.: "CD4 expressed on earliest T-lineage precursor cells in the adult murine thymus." in: Nature, Vol. 349, Issue 6304, pp. 71-4, 1991 (PubMed).
Wu, Antica, Johnson et al.: "Developmental potential of the earliest precursor cells from the adult mouse thymus." in: The Journal of experimental medicine, Vol. 174, Issue 6, pp. 1617-27, 1992 (PubMed).
Janeway: "The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation." in: Annual review of immunology, Vol. 10, pp. 645-74, 1992 (PubMed).
Wineman, Gilmore, Gritzmacher et al.: "CD4 is expressed on murine pluripotent hematopoietic stem cells." in: Blood, Vol. 80, Issue 7, pp. 1717-24, 1992 (PubMed).