anti-Mouse (Murine) CD4 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1172
618
234
68
67
40
36
22
22
19
18
18
16
15
15
12
12
10
10
9
6
5
5
5
4
3
2
2
1
1
1
1
Host
Rat
1324
572
273
10
4
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
258
222
166
100
48
48
46
40
36
34
33
30
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
7
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1675
468
431
332
309
289
152
142
87
60
39
38
30
30
23
19
18
14
14
11
9
7
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118113
$ 621.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118115 FACS IHC (fro) Rat IgG2b Log in to see YTS191-1 15
1 ABIN4292794 FACS ICC IF IHC IHC (fro) Rat IgG kappa Log in to see GK1-5 2
1 ABIN614792 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see GK1-5 7
1 ABIN2480261 IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
1 ABIN255884 FACS IHC (fro) IHC Rat IgG2b Log in to see YTS191-1
1 ABIN2480256 IHC (fro) FACS Rat IgG2b Log in to see YTS191-1 4
1 ABIN476176 FACS IHC (fro) Rat IgG2a, kappa Log in to see RM4-5
1 ABIN476240 FACS IHC (fro) Rat IgG2b, kappa Log in to see GK1-5
1 ABIN2689240 IHC (fro) FACS Biotin Rat IgG2b, kappa Log in to see GK1-5 7
1 ABIN1176974 BR IHC (fro) FACS IP Rat IgG2b, kappa Log in to see GK1-5 9
1 ABIN2689231 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 9
1 ABIN2689230 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see RM4 6
1 ABIN2480258 IHC (fro) FACS Rat IgG2a Log in to see RM4-5 1
1 ABIN2480287 Func IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
1 ABIN1176846 BR CyTox IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 14
1 ABIN133263 FACS IHC (fro) Biotin Rat IgG2a Log in to see CT-CD4
1 ABIN133264 FACS IHC (fro) Biotin Rat IgG2a Log in to see CT-CD4
1 ABIN317082 FACS IHC (fro) Rat IgG2a Log in to see RM4-5
1 ABIN319535 FACS IHC (fro) Rat IgG2b Log in to see YTS191-1
1 ABIN2689224 IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see RM4 13

Top referenced anti-CD4 antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Peptide (BP) 1 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytometry by Time of Flight (CyTOF) 2 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 47 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 483 Antibodies
Functional Studies (Func) 23 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 33 Antibodies
Immunofluorescence (IF) 54 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 128 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1172), (618), (234), (68), (67), (40), (36), (22), (22), (19), (18), (18), (16), (15), (15), (12), (12), (10), (10), (9), (6), (5), (5), (5), (4), (3), (2), (2), (1), (1), (1), (1)
Host Rat
(1324), (572), (273), (10), (4), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(258), (222), (166), (100), (48), (48), (46), (40), (36), (34), (33), (30), (27), (23), (21), (21), (19), (19), (18), (18), (18), (18), (15), (15), (14), (13), (13), (13), (11), (8), (7), (6), (6), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1675), (468), (431), (332), (309), (289), (152), (142), (87), (60), (39), (38), (30), (30), (23), (19), (18), (14), (14), (11), (9), (7), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) Images
Specificity This antibody recognises the CD4 cell surface antigen, expressed by a subset of T lymphocytes. This clone exhibits depleting activity when used in vivo.
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Clone YTS191-1
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 12504
NCBI Accession NP_038516
UniProt P06332
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) Images back to top
Application Notes Flow Cytometry: use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Functional asssays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN118113) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD4 antibody (ABIN118113)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Higgins, McDonald, Whittle, Crockett, Shields, MacDonald et al.: "Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 486-93, 1999

Bemelman, Honey, Adams, Cobbold, Waldmann: "Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2645-8, 1998

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN118113) Staining of mouse spleen with RAT ANTI MOUSE CD4: LOW ENDOTOXIN