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anti-Mouse (Murine) CD4 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Reactivity
Mouse (Murine)
1164
654
241
72
56
41
26
23
22
21
19
15
15
15
11
10
9
8
7
7
6
5
5
5
3
2
2
2
1
1
1
Host
Rat
1330
623
273
8
4
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
274
237
170
106
45
44
42
35
35
35
33
33
24
22
21
20
20
18
17
17
17
17
16
15
15
13
13
8
8
7
7
7
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1742
424
400
333
304
299
181
139
115
68
37
28
28
27
22
18
14
14
11
9
7
6
6
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118115
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008880759 ABIN118113 Func FACS IHC (fro) Rat IgG2b Log in to see YTS191-1 3
0.0008880759 ABIN614792 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see GK1-5 7
0.0008880759 ABIN4292794 FACS ICC IF IHC IHC (fro) Rat IgG kappa Log in to see GK1-5 1
0.0008880759 ABIN255884 FACS IHC (fro) IHC Rat IgG2b Log in to see YTS191-1
0.0008880759 ABIN1176974 BR IHC (fro) FACS IP Rat IgG2b, kappa Log in to see GK1-5 9
0.0008880759 ABIN2689240 IHC (fro) FACS Biotin Rat IgG2b, kappa Log in to see GK1-5 7
0.0008880759 ABIN1176846 BR CyTox IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 14
0.0008880759 ABIN2689230 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see RM4 6
0.0008880759 ABIN2689231 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 9
0.0008880759 ABIN133264 FACS IHC (fro) Biotin Rat IgG2a Log in to see CT-CD4
0.0008880759 ABIN133263 FACS IHC (fro) Biotin Rat IgG2a Log in to see CT-CD4
0.0008880759 ABIN319535 FACS IHC (fro) Rat IgG2b Log in to see YTS191-1
0.0008880759 ABIN317082 FACS IHC (fro) Rat IgG2a Log in to see RM4-5
0.0008880759 ABIN476240 FACS IHC (fro) Rat IgG2b, kappa Log in to see GK1-5
0.0008880759 ABIN476176 FACS IHC (fro) Rat IgG2a, kappa Log in to see RM4-5
0.0008880759 ABIN2480261 IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
0.0008880759 ABIN2689224 IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see RM4 13
0.0008880759 ABIN2689225 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 15
0.0008880759 ABIN2689226 BR IHC (fro) FACS IP Rat IgG2b, kappa Log in to see GK1-5 10
0.0008880759 ABIN133262 FACS IHC (fro) APC Rat IgG2a Log in to see CT-CD4

Top referenced anti-CD4 antibody for Immunohistochemistry (Frozen Sections)

Similar anti-CD4 Antibodies

Application / Reactivity Mouse (Murine)
Biochemical Assay (BCA) 3 Antibodies
Blocking Reagent (BR) 15 Antibodies
Cytotoxicity Test (CyTox) 7 Antibodies
Depletion (Dep) 2 Antibodies
ELISA 49 Antibodies
ELISA (Capture) 2 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 528 Antibodies
Functional Studies (Func) 29 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 44 Antibodies
Immunofluorescence (IF) 69 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 25 Antibodies
Immunohistochemistry (IHC) 108 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 10 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 11 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 69 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)) 5 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1164), (654), (241), (72), (56), (41), (26), (23), (22), (21), (19), (15), (15), (15), (11), (10), (9), (8), (7), (7), (6), (5), (5), (5), (3), (2), (2), (2), (1), (1), (1)
Host Rat
(1330), (623), (273), (8), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(274), (237), (170), (106), (45), (44), (42), (35), (35), (35), (33), (33), (24), (22), (21), (20), (20), (18), (17), (17), (17), (17), (16), (15), (15), (13), (13), (8), (8), (7), (7), (7), (6), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1742), (424), (400), (333), (304), (299), (181), (139), (115), (68), (37), (28), (28), (27), (22), (18), (14), (14), (11), (9), (7), (6), (6), (6), (5), (4), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 15 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN118115) Images
Specificity This antibody recognises the CD4 cell surface antigen, expressed by a subset of T-lymphocytes. This clone exhibits depleting activity when used in vivo (Azide Free CD4 Antibody Cat.-No SM005LE is recommended for this purpose).
Purification Affinity Chromatography on Protein G
Clone YTS191-1
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN118115) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 12504
NCBI Accession NP_038516
UniProt P06332
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN118115) Images back to top
Application Notes Flow Cytometry: Use 10 μL of 1/50-1/200 diluted antibody to label 106 cells.
Immunohistochemistry on Frozen Sections: The epitope recognised by this antibody is reported to be sensitive to Formaldehyde Fixation and tissue processing. The use of Acetone fixation for frozen sections is recommended.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN118115) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-CD4 Antibody (ABIN118115)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Taskin, Sasso, Dimaki, Svendsen, Castillo-León: "Combined cell culture-biosensing platform using vertically aligned patterned peptide nanofibers for cellular studies." in: ACS applied materials & interfaces, Vol. 5, Issue 8, pp. 3323-8, 2013

Abdulreda, Faleo, Molano, Lopez-Cabezas, Molina, Tan, Echeverria, Zahr-Akrawi, Rodriguez-Diaz, Edlund, Leibiger, Bayer, Perez, Ricordi, Caicedo, Pileggi, Berggren: "High-resolution, noninvasive longitudinal live imaging of immune responses." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 31, pp. 12863-8, 2011

Nakashima, Terabe, Berzofsky, Husain, Puri: "A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 187, Issue 10, pp. 4935-46, 2011

Scotland, Stables, Madalli, Watson, Gilroy: "Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice." in: Blood, Vol. 118, Issue 22, pp. 5918-27, 2011

Eller, Wolf, Huber, Metz, Mayer, McKenzie, Maurer, Rosenkranz, Wolf: "IL-9 production by regulatory T cells recruits mast cells that are essential for regulatory T cell-induced immune suppression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 186, Issue 1, pp. 83-91, 2010

Grimm, Gasser, Bueter, Strehl, Wang, Nichiporuk, Meyer, Germer, Waaga-Gasser, Thalheimer: "Evaluation of immunological escape mechanisms in a mouse model of colorectal liver metastases." in: BMC cancer, Vol. 10, pp. 82, 2010

Huber, Tagwerker, Heininger, Mayer, Rosenkranz, Eller: "The proteasome inhibitor bortezomib aggravates renal ischemia-reperfusion injury." in: American journal of physiology. Renal physiology, Vol. 297, Issue 2, pp. F451-60, 2009

Gaupp, Cannella, Raine: "Amelioration of experimental autoimmune encephalomyelitis in IL-4Ralpha-/- mice implicates compensatory up-regulation of Th2-type cytokines." in: The American journal of pathology, Vol. 173, Issue 1, pp. 119-29, 2008

Jégou, Chan, Schouft, Griffiths, Neal, Gasque, Vaudry, Fontaine: "C3d binding to the myelin oligodendrocyte glycoprotein results in an exacerbated experimental autoimmune encephalomyelitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 178, Issue 5, pp. 3323-31, 2007

Wolf, Hochegger, Wolf, Rumpold, Gastl, Tilg, Mayer, Gunsilius, Rosenkranz: "CD4+CD25+ regulatory T cells inhibit experimental anti-glomerular basement membrane glomerulonephritis in mice." in: Journal of the American Society of Nephrology : JASN, Vol. 16, Issue 5, pp. 1360-70, 2005

Gardinier, Khoury, Lehn: "Enforced helicity: efficient access to self-organized helical molecular strands by the imine route." in: Chemistry (Weinheim an der Bergstrasse, Germany), Vol. 6, Issue 22, pp. 4124-31, 2000

Higgins, McDonald, Whittle, Crockett, Shields, MacDonald et al.: "Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 486-93, 1999

Bemelman, Honey, Adams, Cobbold, Waldmann: "Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2645-8, 1998

Bourmeyster, Stasia, Garin, Gagnon, Boquet, Vignais: "Copurification of rho protein and the rho-GDP dissociation inhibitor from bovine neutrophil cytosol. Effect of phosphoinositides on rho ADP-ribosylation by the C3 exoenzyme of Clostridium botulinum." in: Biochemistry, Vol. 31, Issue 51, pp. 12863-9, 1993

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN118115) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (ABIN118115) Staining of mouse spleen cells with Rat anti Mouse CD4 (ABIN118115)