anti-Mouse (Murine) CD4 antibody for Immunohistochemistry (Frozen Sections)

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Reactivity
Mouse (Murine)
1220
631
234
68
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Rat
1377
586
281
11
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
1743
490
445
352
323
309
157
155
88
77
39
38
31
30
30
25
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN118113
$ 621.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN118115 FACS IHC (fro) Rat IgG2b Log in to see YTS191-1 15
1 ABIN4292794 FACS ICC IF IHC IHC (fro) Rat IgG kappa Log in to see GK1-5 3
1 ABIN614792 Func IHC (fro) FACS IF IP Rat IgG2b Log in to see GK1-5 7
1 ABIN2480261 IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
1 ABIN255884 FACS IHC (fro) IHC Rat IgG2b Log in to see YTS191-1
1 ABIN2480256 IHC (fro) FACS Rat IgG2b Log in to see YTS191-1 4
1 ABIN1858300 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 27-394 Log in to see
1 ABIN2689240 IHC (fro) FACS Biotin Rat IgG2b, kappa Log in to see GK1-5 7
1 ABIN476240 FACS IHC (fro) Rat IgG2b, kappa Log in to see GK1-5
1 ABIN476176 FACS IHC (fro) Rat IgG2a, kappa Log in to see RM4-5
1 ABIN1176974 BR IHC (fro) FACS IP Rat IgG2b, kappa Log in to see GK1-5 9
1 ABIN2689231 BR IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 9
1 ABIN2689230 IHC (f) IHC (fro) FACS IHC (zinc) Rat IgG2a, kappa Log in to see RM4 6
1 ABIN2480258 IHC (fro) FACS Rat IgG2a Log in to see RM4-5 1
1 ABIN2480287 Func IHC (fro) FACS IF IP ELISA WB Rat IgG2b Log in to see GK1-5 4
1 ABIN1176846 BR CyTox IHC (f) IHC (fro) FACS IHC (zinc) IP Rat IgG2a, kappa Log in to see H129-19 14
1 ABIN206563 FACS IHC (fro) IF IP ELISA Mouse IgG2a Log in to see RIV6
1 ABIN133264 FACS IHC (fro) Biotin Rat IgG2a Log in to see CT-CD4
1 ABIN133263 FACS IHC (fro) Biotin Rat IgG2a Log in to see CT-CD4
1 ABIN317082 FACS IHC (fro) Rat IgG2a Log in to see RM4-5

General

Antigen CD4 Molecule (CD4) Antibodies
Reactivity Mouse (Murine)
(1220), (631), (234), (68), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Rat
(1377), (586), (281), (11), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (170), (103), (49), (48), (46), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
(1743), (490), (445), (352), (323), (309), (157), (155), (88), (77), (39), (38), (31), (30), (30), (25), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) Images
Specificity This antibody recognises the CD4 cell surface antigen, expressed by a subset of T lymphocytes. This clone exhibits depleting activity when used in vivo.
Purification Affinity chromatography on Protein G
Endotoxin Level Low Endotoxin
Clone YTS191-1
Isotype IgG2b

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 12504
NCBI Accession NP_038516
UniProt P06332
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) Images back to top
Application Notes Flow Cytometry: use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Functional asssays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN118113) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4 without preservatives.
Preservative Without preservative
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD4 antibody (ABIN118113)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Higgins, McDonald, Whittle, Crockett, Shields, MacDonald et al.: "Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 486-93, 1999

Bemelman, Honey, Adams, Cobbold, Waldmann: "Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2645-8, 1998

Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN118113) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 Molecule (CD4) antibody (ABIN118113) Staining of mouse spleen with RAT ANTI MOUSE CD4: LOW ENDOTOXIN