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|Application / Reactivity||Mouse (Murine)|
|Biochemical Assay (BCA)||3 Antibodies|
|Blocking Reagent (BR)||15 Antibodies|
|Cytotoxicity Test (CyTox)||7 Antibodies|
|Depletion (Dep)||2 Antibodies|
|ELISA (Capture)||2 Antibodies|
|Enzyme Immunoassay (EIA)||3 Antibodies|
|Flow Cytometry (FACS)||469 Antibodies|
|Functional Studies (Func)||29 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Immunocytochemistry (ICC)||35 Antibodies|
|Immunofluorescence (IF)||64 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||100 Antibodies|
|Immunohistochemistry (Acetone-fixed) (IHC (af))||10 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||7 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||56 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||15 Antibodies|
|Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc))||5 Antibodies|
|Antigen||CD4 Molecule (CD4) Antibodies|
|Reactivity||Mouse (Murine) Alternatives|
|Conjugate||This CD4 antibody is un-conjugated Alternatives|
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
|3 references available|
|Supplier||Log in to see|
Product Details anti-CD4 AntibodyTarget Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN118113) Images
|Specificity||This antibody recognises the CD4 cell surface antigen, expressed by a subset of T lymphocytes. This clone exhibits depleting activity when used in vivo.|
|Purification||Affinity chromatography on Protein G|
|Endotoxin Level||Low Endotoxin|
Target Details CD4Product Details anti-CD4 Antibody Application Details Handling References for anti-CD4 Antibody (ABIN118113) Images back to top
|Alternative Name||CD4 (CD4 Antibody Abstract)|
|Background||CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45 % of peripheral blood lymphocytes. It is also present on 80 % of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4|
|Research Area||Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells|
Application DetailsProduct Details anti-CD4 Antibody Target Details CD4 Handling References for anti-CD4 Antibody (ABIN118113) Images back to top
Flow Cytometry: use 10 μL of 1/100-1/200 diluted antibody to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen Sections. Functional asssays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD4 Antibody Target Details CD4 Application Details References for anti-CD4 Antibody (ABIN118113) Images back to top
|Buffer||PBS, pH 7.4 without preservatives.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.|
References for anti-CD4 Antibody (ABIN118113)Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Higgins, McDonald, Whittle et al.: "Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 486-93, 1999 (PubMed).
Bemelman, Honey, Adams et al.: "Bone marrow transplantation induces either clonal deletion or infectious tolerance depending on the dose." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 160, Issue 6, pp. 2645-8, 1998 (PubMed).
Cobbold, Martin, Waldmann: "The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies." in: European journal of immunology, Vol. 20, Issue 12, pp. 2747-55, 1991 (PubMed).
ImagesProduct Details anti-CD4 Antibody Target Details CD4 Application Details Handling References for anti-CD4 Antibody (ABIN118113) back to top