anti-Rat (Rattus) CD4 antibody for Flow Cytometry

Recommended CD4 Antibody (supplied by: Log in to see )

Antigen
CD4 Molecule (CD4) Antibodies
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Epitope
Domain 1
66
64
20
18
15
15
15
13
12
10
9
8
8
8
8
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
1219
632
233
69
67
40
36
27
26
22
21
19
18
16
15
15
13
10
10
9
7
6
5
5
5
4
4
3
3
3
3
2
2
2
2
2
1
1
1
1
Host
Mouse
1378
587
280
10
4
1
1
1
Clonality
Monoclonal
Conjugate
This CD4 antibody is un-conjugated
266
227
170
103
49
48
46
40
36
34
34
30
27
25
21
21
19
19
18
18
18
18
16
15
15
13
13
13
11
9
9
8
6
5
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
1743
490
442
353
321
310
156
155
87
77
39
38
30
30
25
19
18
14
14
11
9
9
6
6
5
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN120378
$ 687.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.317143 ABIN2480218 FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 4
13.317143 ABIN2660683 FACS APC-Cy7 Mouse IgG1 kappa Log in to see W3-25 7
13.317143 ABIN2663441 FACS PE Mouse IgG2a kappa Log in to see OX-35 2
13.317143 ABIN2663438 FACS PE Mouse IgG1 kappa Log in to see W3-25 6
13.317143 ABIN2657779 FACS Alexa Fluor 647 Mouse IgG2a kappa Log in to see OX-35 2
13.317143 ABIN2657776 FACS Alexa Fluor 647 Mouse IgG1 kappa Log in to see W3-25 6
13.317143 ABIN2657419 FACS Alexa Fluor 488 Mouse IgG1 kappa Log in to see W3-25 7
13.317143 ABIN2661701 FACS IP IHC WB FITC Mouse IgG1 kappa Log in to see W3-25 6
13.317143 ABIN2661706 FACS IHC FITC Mouse IgG2a kappa Log in to see OX-38 1
13.317143 ABIN2658653 FACS APC Mouse IgG1 kappa Log in to see W3-25 6
13.317143 ABIN2659510 FACS PE-Cy7 Mouse IgG1 kappa Log in to see W3-25 6
13.317143 ABIN2480228 IHC (fro) FACS IP Mouse IgG2a Domain 1 Log in to see OX-38 4
13.317143 ABIN5075610 FACS IHC IHC (fro) IHC (p) Mouse IgG1 Log in to see W3-25
13.317143 ABIN4900071 FACS WB Sheep IgG AA 28-394 Log in to see Polyclonal
13.317143 ABIN133174 FACS Mouse IgG1 Log in to see W3-25
1 ABIN120377 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN120376 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN111996 Func FACS IHC (fro) Mouse IgG2a Log in to see Rib 5-2 2
1 ABIN120372 Func FACS IHC (fro) IHC (p) Mouse IgG1 Domain 1 Log in to see W3-25 9
1 ABIN120107 FACS IHC (fro) Mouse IgG2a Domain 1 Log in to see OX-38 3

Top referenced anti-CD4 antibody for Flow Cytometry

Similar anti-CD4 Antibodies

Application / Reactivity Rat (Rattus)
Blocking Reagent (BR) 2 Antibodies
Cytotoxicity Test (CyTox) 4 Antibodies
ELISA 15 Antibodies
ELISA (Capture) 1 Antibodies
ELISpot (Capture) (ELISPOT (Capture)) 1 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
Flow Cytometry (FACS) 156 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 15 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 35 Antibodies
Immunoprecipitation (IP) 20 Antibodies

General

Antigen CD4 Molecule (CD4) Antibodies
Epitope Domain 1
(66), (64), (20), (18), (15), (15), (15), (13), (12), (10), (9), (8), (8), (8), (8), (6), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(1219), (632), (233), (69), (67), (40), (36), (27), (26), (22), (21), (19), (18), (16), (15), (15), (13), (10), (10), (9), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Host Mouse
(1378), (587), (280), (10), (4), (1), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CD4 antibody is un-conjugated
(266), (227), (170), (103), (49), (48), (46), (40), (36), (34), (34), (30), (27), (25), (21), (21), (19), (19), (18), (18), (18), (18), (16), (15), (15), (13), (13), (13), (11), (9), (9), (8), (6), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(1743), (490), (442), (353), (321), (310), (156), (155), (87), (77), (39), (38), (30), (30), (25), (19), (18), (14), (14), (11), (9), (9), (6), (6), (5), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 9 references available
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Product Details anti-CD4 Antibody

Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120378) Images
Purification Affinity chromatography on Protein A
Immunogen Rat Thymocyte Membrane Glycoproteins. Spleen cells from immunised BALB/c mice were fused with cells of the NS-1 mouse myeloma cell line.
Clone W3-25
Isotype IgG1

Target Details CD4

Product Details anti-CD4 Antibody Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120378) Images back to top
Antigen
Alternative Name CD4 (CD4 Antibody Abstract)
Background CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes ("helper/inducer" T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4
Gene ID 10116
UniProt P05540
Research Area Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events

Application Details

Product Details anti-CD4 Antibody Target Details CD4 Handling ProductDetails: References for anti-CD4 antibody (ABIN120378) Images back to top
Application Notes Flow cytometry: 1/100 - 1/200, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen and paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CD4 Antibody Target Details CD4 Application Details ProductDetails: References for anti-CD4 antibody (ABIN120378) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CD4 antibody (ABIN120378)

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling Images back to top
Background publications

Hofmann, Lachnit, Streppel, Witter, Neiss, Guntinas-Lichius, Angelov: "Increased expression of ICAM-1, VCAM-1, MCP-1, and MIP-1 alpha by spinal perivascular macrophages during experimental allergic encephalomyelitis in rats." in: BMC immunology, Vol. 3, pp. 11, 2003

Whiteland, Nicholls, Shimeld, Easty, Williams, Hill: "Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 43, Issue 3, pp. 313-20, 1995

Pelegrí, Franch, Castellote, Castell: "Immunohistochemical changes in synovial tissue during the course of adjuvant arthritis." in: The Journal of rheumatology, Vol. 22, Issue 1, pp. 124-32, 1995

Clark, Jefferies, Barclay, Gagnon, Williams: "Peptide and nucleotide sequences of rat CD4 (W3/25) antigen: evidence for derivation from a structure with four immunoglobulin-related domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 6, pp. 1649-53, 1987

Cantrell, Robins, Baldwin: "Rat lymphocyte subsets: cellular requirements for the generation of T-cell growth factor." in: Cellular immunology, Vol. 70, Issue 2, pp. 367-72, 1982

Barclay: "The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues." in: Immunology, Vol. 42, Issue 4, pp. 593-600, 1981

Mason, Pugh, Webb: "The rat mixed lymphocyte reaction: roles of a dendritic cell in intestinal lymph and T-cell subsets defined by monoclonal antibodies." in: Immunology, Vol. 44, Issue 1, pp. 75-87, 1981

Brideau, Carter, McMaster, Mason, Williams: "Two subsets of rat T lymphocytes defined with monoclonal antibodies." in: European journal of immunology, Vol. 10, Issue 8, pp. 609-15, 1980

Williams, Galfrè, Milstein: "Analysis of cell surfaces by xenogeneic myeloma-hybrid antibodies: differentiation antigens of rat lymphocytes." in: Cell, Vol. 12, Issue 3, pp. 663-73, 1978

Images

Product Details anti-CD4 Antibody Target Details CD4 Application Details Handling ProductDetails: References for anti-CD4 antibody (ABIN120378) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-CD4 antibody (CD4 Molecule) (Domain 1) (ABIN120378) Staining of stimulated rat spleen cells with MOUSE ANTI RAT CD4 (ABIN120378).