anti-Human CREB1 antibody for Enzyme Immunoassay

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • creb
  • CREB1
  • Creb1
  • CREB
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • Creb-1
  • creb1
  • zgc:55598
  • cAMP responsive element binding protein 1
  • cAMP responsive element binding protein 1a
  • cyclic AMP response element binding protein
  • CREB1
  • creb1
  • Creb1
  • LOC100351999
  • creb1a
  • CREB
Epitope
pSer133
141
38
38
37
22
20
16
15
15
9
8
7
7
6
6
5
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
548
329
299
41
38
36
30
27
26
26
19
17
15
12
7
7
7
2
1
1
1
1
Host
Rabbit
449
101
11
1
Clonality
Polyclonal
Conjugate
This CREB1 antibody is un-conjugated
18
15
15
7
7
6
6
6
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
441
225
192
100
91
48
44
42
31
11
10
10
4
3
3
2
2
1
1
1
Supplier
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Catalog No. ABIN358113
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN116948 EMSA EIA IP WB Rabbit N-Term Log in to see Polyclonal 1
1 ABIN126754 EIA WB Mouse IgG1 pSer133 Log in to see 1-00E-010
1 ABIN5556215 EIA FACS IF WB Rabbit Ig Fraction AA 112-140, Middle Region Log in to see Polyclonal
1 ABIN5556654 EIA IP WB Rabbit pSer133 Log in to see Polyclonal
1 ABIN265371 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN782354 EIA IHC (p) WB Mouse IgG2a AA 1-342 Log in to see 5F2
1 ABIN782353 EIA IF IHC (p) WB Mouse IgG1 AA 1-342 Log in to see 3F1-1B2
1 ABIN1106831 EIA IF IHC (p) WB Mouse IgG1 Log in to see 5G3 4
1 ABIN452620 EIA Mouse IgG2b pSer133 Log in to see B1C4
1 ABIN452618 EIA Mouse IgG1 pSer133 Log in to see A3A4

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Epitope pSer133
(141), (38), (38), (37), (22), (20), (16), (15), (15), (9), (8), (7), (7), (6), (6), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(548), (329), (299), (41), (38), (36), (30), (27), (26), (26), (19), (17), (15), (12), (7), (7), (7), (2), (1), (1), (1), (1)
Host Rabbit
(449), (101), (11), (1)
Clonality
Polyclonal
Conjugate This CREB1 antibody is un-conjugated
(18), (15), (15), (7), (7), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(441), (225), (192), (100), (91), (48), (44), (42), (31), (11), (10), (10), (4), (3), (3), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling References for anti-CREB1 antibody (ABIN358113) Images
Specificity This antibody detects CREB pSer133. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S133 of human CREB.
Isotype Ig

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling References for anti-CREB1 antibody (ABIN358113) Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background CREB is a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, (CRE), a sequence present in many viral and cellular promoters. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway.Synonyms: CREB-1, Cyclic AMP-responsive element-binding protein 1, cAMP-responsive element-binding protein 1
Molecular Weight 36688 Da
Gene ID 1385, 9606
UniProt P16220
Pathways TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Cell Size, Toll-Like Receptors Cascades, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Positive Regulation of fat Cell Differentiation

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling References for anti-CREB1 antibody (ABIN358113) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details References for anti-CREB1 antibody (ABIN358113) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CREB1 antibody (ABIN358113)

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling Images back to top
Background publications

Samten, Howard, Weis, Wu, Shams, Townsend, Safi, Barnes: "Cyclic AMP response element-binding protein positively regulates production of IFN-gamma by T cells in response to a microbial pathogen." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 174, Issue 10, pp. 6357-63, 2005

Zhang, Odom, Koo, Conkright, Canettieri, Best, Chen, Jenner, Herbolsheimer, Jacobsen, Kadam, Ecker, Emerson, Hogenesch, Unterman, Young, Montminy: "Genome-wide analysis of cAMP-response element binding protein occupancy, phosphorylation, and target gene activation in human tissues." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 102, Issue 12, pp. 4459-64, 2005

Cho, Choi, Kim, Baek, Chung, Moon, Kim, Lee, Koo, Yoon: "Prostaglandin E2 induces MUC8 gene expression via a mechanism involving ERK MAPK/RSK1/cAMP response element binding protein activation in human airway epithelial cells." in: The Journal of biological chemistry, Vol. 280, Issue 8, pp. 6676-81, 2005

Bailey, Tyson-Capper, Gilmore, Robson, Europe-Finner: "Identification of human myometrial target genes of the cAMP pathway: the role of cAMP-response element binding (CREB) and modulator (CREMalpha and CREMtau2alpha) proteins." in: Journal of molecular endocrinology, Vol. 34, Issue 1, pp. 1-17, 2005

Perry, Sklan, Soreq: "CREB regulates AChE-R-induced proliferation of human glioblastoma cells." in: Neoplasia (New York, N.Y.), Vol. 6, Issue 3, pp. 279-86, 2004

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling References for anti-CREB1 antibody (ABIN358113) back to top
Supplier Images
 image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody (ABIN358113) anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody
 image for anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody (ABIN358113) anti-CAMP Responsive Element Binding Protein 1 (CREB1) (pSer133) antibody (Image 2)