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anti-Human CREB1 antibody for Gel Shift

Recommended CREB1 Antibody (supplied by: Log in to see )

Antigen
CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
  • 2310001E10Rik
  • 3526402H21Rik
  • AV083133
  • Creb
  • CREB
  • creb
  • Creb-1
  • creb1
  • Creb1
  • CREB1
  • zgc:55598
Reactivity
Human, Mouse (Murine), Rat (Rattus)
469
231
224
27
25
19
18
15
14
9
9
9
2
2
1
1
Host
Rabbit
405
70
10
1
Clonality
Monoclonal
Conjugate
This CREB1 antibody is un-conjugated
15
14
13
7
6
6
5
4
3
3
3
3
3
3
3
Application
Gel Shift (GS), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
372
216
184
67
58
36
35
28
26
11
9
3
3
2
2
1
1
1
Supplier
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Available images

Catalog No. ABIN189266
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008757905 ABIN269747 GS ELISA WB Rabbit IgG N-Term, AA 5-45 Log in to see Polyclonal

Similar anti-CREB1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 1 Antibodies 1 Antibodies
ELISA 71 Antibodies 72 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 3 Antibodies
Gel Shift (GS) 2 Antibodies
Immunochromatography (IC) 4 Antibodies
Immunocytochemistry (ICC) 13 Antibodies
Immunofluorescence (IF) 28 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 114 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 19 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Western Blotting (WB) 159 Antibodies

General

Antigen CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(469), (231), (224), (27), (25), (19), (18), (15), (14), (9), (9), (9), (2), (2), (1), (1)
Host Rabbit
(405), (70), (10), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CREB1 antibody is un-conjugated
(15), (14), (13), (7), (6), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3)
Application Gel Shift (GS), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(372), (216), (184), (67), (58), (36), (35), (28), (26), (11), (9), (3), (3), (2), (2), (1), (1), (1)
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Product Details anti-CREB1 Antibody

Target Details CREB1 Application Details Handling Images
Specificity This antibody can also recognize the splice isoform of CREB.
Cross-Reactivity (Details) Mouse cross reactivity tested by western blot,IHC. rat,cross reactivity tested by western blot only. Predicted to cross-react with bovine, based on sequence identity.
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen.
Clone E306
Isotype IgG

Target Details CREB1

Product Details anti-CREB1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CREB1 (CREB1 Antibody Abstract)
Background CREB is a transcription factor protein that binds the cAMP response element (CRE),thereby stimulating transcription of this sequence. CREB plays a role in propagatingnumerous extracellular signals to the nuclues (1-3). CREB is activated byphosphorylation at the Ser133 position by various signaling pathways, and some of thekinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV andMAPKAPK-2 (1-3). Alternate Names: anti-CREB 1 antibody, anti-CREB1 antibody, anti-MGC9284 antibody,anti-Transactivator protein antibody, anti-cAMP response element binding proteinantibody, anti-cAMP responsive element binding protein 1 antibody.
Gene Symbol: CREB1
Gene ID 1385, 12912, 81646
Pathways TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CREB1 Antibody Target Details CREB1 Handling Images back to top
Application Notes Recommended dilutions: Flow Cytometry 1:40, Gel Super Shift Assays 1:1-1:100, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:80, Western Blot 1:500-1000
Protocol Protocol specific for CREB Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (ABIN189266) Western blot analysis on A431 cell lysate using anti-CREB RabMAb (ABIN189266), diluti...
Immunohistochemistry (IHC) image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (ABIN189266) Immunohistochemical analysis of paraffin-embedded prostate carcinoma using anti-CREB ...
Immunofluorescence (IF) image for anti-CREB1 antibody (CAMP Responsive Element Binding Protein 1) (ABIN189266) Immunofluorescent staining of PC-12 cells using anti-CREB RabMAb