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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Dot Blot (DB)||1 Antibodies||1 Antibodies|
|Electrophoretic Mobility-Shift Assay (EMSA)||1 Antibodies||1 Antibodies|
|ELISA||71 Antibodies||72 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Flow Cytometry (FACS)||3 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunochromatography (IC)||4 Antibodies|
|Immunocytochemistry (ICC)||13 Antibodies|
|Immunofluorescence (IF)||28 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||36 Antibodies|
|Immunohistochemistry (IHC)||114 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||29 Antibodies|
|Immunoprecipitation (IP)||19 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Western Blotting (WB)||159 Antibodies|
|Antigen||CAMP Responsive Element Binding Protein 1 (CREB1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This CREB1 antibody is un-conjugated Alternatives|
Gel Shift (GS), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-CREB1 AntibodyTarget Details CREB1 Application Details Handling Images
|Specificity||This antibody can also recognize the splice isoform of CREB.|
|Cross-Reactivity (Details)||Mouse cross reactivity tested by western blot,IHC. rat,cross reactivity tested by western blot only. Predicted to cross-react with bovine, based on sequence identity.|
|Immunogen||A synthetic peptide corresponding to residues before DNA binding motif of human CREB was used as immunogen.|
Target Details CREB1Product Details anti-CREB1 Antibody Application Details Handling Images back to top
|Alternative Name||CREB1 (CREB1 Antibody Abstract)|
CREB is a transcription factor protein that binds the cAMP response element (CRE),thereby stimulating transcription of this sequence. CREB plays a role in propagatingnumerous extracellular signals to the nuclues (1-3). CREB is activated byphosphorylation at the Ser133 position by various signaling pathways, and some of thekinases involved in phosphorylating CREB at Ser133 are p90RSK, MSK, CaMKIV andMAPKAPK-2 (1-3). Alternate Names: anti-CREB 1 antibody, anti-CREB1 antibody, anti-MGC9284 antibody,anti-Transactivator protein antibody, anti-cAMP response element binding proteinantibody, anti-cAMP responsive element binding protein 1 antibody.
Gene Symbol: CREB1
|Gene ID||1385, 12912, 81646|
|Pathways||TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CREB1 Antibody Target Details CREB1 Handling Images back to top
|Application Notes||Recommended dilutions: Flow Cytometry 1:40, Gel Super Shift Assays 1:1-1:100, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:250-500, Immunoprecipitation 1:80, Western Blot 1:500-1000|
Protocol specific for CREB Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-CREB1 Antibody Target Details CREB1 Application Details Handling back to top