anti-Human FYB antibody for Immunofluorescence

Recommended FYB Antibody (supplied by: Log in to see )

Antigen
FYN-Binding Protein (FYB) Antibodies
  • fyb
  • ADAP
  • PRO0823
  • SLAP-130
  • B630013F22Rik
  • FYB-120/130
  • Slap130
  • p120/130
  • p120/p130
  • RGD1563421
  • FYN-binding protein
  • FYN binding protein
  • fyb
  • FYB
  • Fyb
Epitope
AA 673-783
21
9
9
7
4
2
2
1
1
1
1
Reactivity
Human
90
27
21
6
5
4
3
3
2
2
Host
Mouse
59
21
9
2
Clonality
Monoclonal
Conjugate
This FYB antibody is un-conjugated
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
63
39
13
12
10
5
4
4
4
2
1
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Catalog No. ABIN968265
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN968266 IF WB Mouse IgG1 AA 673-783 Log in to see 5-FYB 4
1 ABIN5554508 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN5554509 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2196227 IC IF ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2196229 IC IF ELISA WB Rabbit IgG N-Term Log in to see Polyclonal

General

Antigen FYN-Binding Protein (FYB) Antibodies
Epitope AA 673-783
(21), (9), (9), (7), (4), (2), (2), (1), (1), (1), (1)
Reactivity Human
(90), (27), (21), (6), (5), (4), (3), (3), (2), (2)
Host Mouse
(59), (21), (9), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FYB antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(63), (39), (13), (12), (10), (5), (4), (4), (4), (2), (1)
Pubmed 4 references available
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Product Details anti-FYB Antibody

Target Details FYB Application Details Handling References for anti-FYB antibody (ABIN968265) Images
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Immunogen Human FYB aa. 673-783
Clone 5-FYB
Isotype IgG1

Target Details FYB

Product Details anti-FYB Antibody Application Details Handling References for anti-FYB antibody (ABIN968265) Images back to top
Antigen
Alternative Name FYB (FYB Antibody Abstract)
Background Engagement of the T cell receptor (TcR) results in the activation of multiple intracellular src protein tyrosine kinases (PTKs) including p56 [lck] and p59 [fyn]. PTK activation induces TcRzeta phosphorylation and the subsequent recruitment and activation of ZAP-70 which specifically phosphorylates SLP-76. SLP-76 interacts with multiple components of T cell signaling pathways, including Grb-2, PLC-gamma, Vav, and SLAP-130 (also known as SLP-76 Associated Phosphoprotein of 130 kDa). SLAP-130 contains multiple tyrosine based motifs, proline base type I and II SH3 domain binding motifs, lysine/glutamic acid rich nuclear localization motifs, and an SH3-like domain. It associates with the SH2 domain of SLP-76 and is phosphorylated by TcR induced PTKs. This protein has also been reported to directly interact with fyn and has been referred to as fyn binding protein (FYB). Co-transfection data has shown that a limited concentration of FYB/SLAP-130 enhances TcR-induced IL-2 production, while overexpression downregulates gene expression. Thus, FYB/SLAP-130 is viewed to be a regulatory component of the fyn/SLP-76 signaling cascade in T cells.
Synonyms: Fyn Binding Protein, SLAP-130
Molecular Weight 130 kDa
Pathways TCR Signaling, Protein targeting to Nucleus

Application Details

Product Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB antibody (ABIN968265) Images back to top
Comment

Related Products: ABIN968537, ABIN967389

Restrictions For Research Use only

Handling

Product Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB antibody (ABIN968265) Images back to top
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20° C.

References for anti-FYB antibody (ABIN968265)

Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
Product cited in:

Geng, Pfister, Kraeft, Rudd: "Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: differential involvement of the FYB SH3 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11527-32, 2001

Oda, Ikeda, Ochs, Druker, Ozaki, Handa, Ariga, Sakiyama, Witte, Wahl: "Rapid tyrosine phosphorylation and activation of Bruton's tyrosine/Tec kinases in platelets induced by collagen binding or CD32 cross-linking." in: Blood, Vol. 95, Issue 5, pp. 1663-70, 2000

da Silva, Li, de Vera, Canto, Findell, Rudd: "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 14, pp. 7493-8, 1997

Musci, Hendricks-Taylor, Motto, Paskind, Kamens, Turck, Koretzky: "Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases." in: The Journal of biological chemistry, Vol. 272, Issue 18, pp. 11674-7, 1997

Images

Product Details anti-FYB Antibody Target Details FYB Application Details Handling References for anti-FYB antibody (ABIN968265) back to top
Supplier Images
Western Blotting (WB) image for anti-FYN-Binding Protein (FYB) (AA 673-783) antibody (ABIN968265) Western blot analysis of FYB on a Jurkat cell lysate (Human T-cell leukemia, ATCC TIB...