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|Antigen||FYN-Binding Protein (FYB) Antibodies|
|Epitope||AA 673-783 Alternatives|
|Conjugate||This FYB antibody is un-conjugated Alternatives|
Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
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Product Details anti-FYB AntibodyTarget Details FYB Application Details Handling References for anti-FYB antibody (ABIN968265) Images
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Human FYB aa. 673-783|
Target Details FYBProduct Details anti-FYB Antibody Application Details Handling References for anti-FYB antibody (ABIN968265) Images back to top
|Alternative Name||FYB (FYB Antibody Abstract)|
Engagement of the T cell receptor (TcR) results in the activation of multiple intracellular src protein tyrosine kinases (PTKs) including p56 [lck] and p59 [fyn]. PTK activation induces TcRzeta phosphorylation and the subsequent recruitment and activation of ZAP-70 which specifically phosphorylates SLP-76. SLP-76 interacts with multiple components of T cell signaling pathways, including Grb-2, PLC-gamma, Vav, and SLAP-130 (also known as SLP-76 Associated Phosphoprotein of 130 kDa). SLAP-130 contains multiple tyrosine based motifs, proline base type I and II SH3 domain binding motifs, lysine/glutamic acid rich nuclear localization motifs, and an SH3-like domain. It associates with the SH2 domain of SLP-76 and is phosphorylated by TcR induced PTKs. This protein has also been reported to directly interact with fyn and has been referred to as fyn binding protein (FYB). Co-transfection data has shown that a limited concentration of FYB/SLAP-130 enhances TcR-induced IL-2 production, while overexpression downregulates gene expression. Thus, FYB/SLAP-130 is viewed to be a regulatory component of the fyn/SLP-76 signaling cascade in T cells.
Synonyms: Fyn Binding Protein, SLAP-130
|Molecular Weight||130 kDa|
|Pathways||TCR Signaling, Protein targeting to Nucleus|
Application DetailsProduct Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB antibody (ABIN968265) Images back to top
Related Products: ABIN968537, ABIN967389
|Restrictions||For Research Use only|
HandlingProduct Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB antibody (ABIN968265) Images back to top
|Buffer||Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.|
|Precaution of Use|
|Storage Comment||Store undiluted at -20° C.|
References for anti-FYB antibody (ABIN968265)Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
|Product cited in:||
Geng, Pfister, Kraeft, Rudd: "Adaptor FYB (Fyn-binding protein) regulates integrin-mediated adhesion and mediator release: differential involvement of the FYB SH3 domain." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11527-32, 2001
Oda, Ikeda, Ochs, Druker, Ozaki, Handa, Ariga, Sakiyama, Witte, Wahl: "Rapid tyrosine phosphorylation and activation of Bruton's tyrosine/Tec kinases in platelets induced by collagen binding or CD32 cross-linking." in: Blood, Vol. 95, Issue 5, pp. 1663-70, 2000
da Silva, Li, de Vera, Canto, Findell, Rudd: "Cloning of a novel T-cell protein FYB that binds FYN and SH2-domain-containing leukocyte protein 76 and modulates interleukin 2 production." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 14, pp. 7493-8, 1997
Musci, Hendricks-Taylor, Motto, Paskind, Kamens, Turck, Koretzky: "Molecular cloning of SLAP-130, an SLP-76-associated substrate of the T cell antigen receptor-stimulated protein tyrosine kinases." in: The Journal of biological chemistry, Vol. 272, Issue 18, pp. 11674-7, 1997
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