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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||7 Antibodies||39 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||4 Antibodies|
|Immunocytochemistry (ICC)||5 Antibodies||14 Antibodies|
|Immunofluorescence (IF)||1 Antibodies||6 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||3 Antibodies|
|Immunoprecipitation (IP)||2 Antibodies|
|Western Blotting (WB)||14 Antibodies||59 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies|
|Immunochromatography (IC)||2 Antibodies|
|Antigen||FYN-Binding Protein (FYB) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This FYB antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
|4 references available|
|Supplier||Log in to see|
Product Details anti-FYB AntibodyTarget Details FYB Application Details Handling References for anti-FYB Antibody (ABIN499215) Images
|Specificity||This antibody recognizes ADAP (C-term).|
|Purification||Affinity chromatography purified via peptide column|
|Immunogen||A 20 amino acid peptide from near the carboxy terminus of Human ADAP|
Target Details FYBProduct Details anti-FYB Antibody Application Details Handling References for anti-FYB Antibody (ABIN499215) Images back to top
|Alternative Name||FYN-Binding Protein (FYB Antibody Abstract)|
|Background||The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.Synonyms: Adhesion and degranulation promoting adaptor protein, FYB-120/130, FYN-T-binding protein, SLAP-130, SLAP130, SLP-76-associated phosphoprotein, p120/p130|
Application DetailsProduct Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB Antibody (ABIN499215) Images back to top
ELISA. Western Blot: ADAP antibody can be used for detection of ADAP at 0.5 - 1 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB Antibody (ABIN499215) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-FYB Antibody (ABIN499215)Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
Mueller, Thomas, Burbach et al.: "Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3559-69, 2007 (PubMed).
Medeiros, Burbach, Mueller et al.: "Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1." in: Science (New York, N.Y.), Vol. 316, Issue 5825, pp. 754-8, 2007 (PubMed).
Rawlings, Sommer, Moreno-García: "The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes." in: Nature reviews. Immunology, Vol. 6, Issue 11, pp. 799-812, 2006 (PubMed).
Griffiths, Krawczyk, Kong et al.: "Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap." in: Science (New York, N.Y.), Vol. 293, Issue 5538, pp. 2260-3, 2001 (PubMed).
ImagesProduct Details anti-FYB Antibody Target Details FYB Application Details Handling References for anti-FYB Antibody (ABIN499215) back to top