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anti-Mouse (Murine) FYB antibody for Western Blotting

Recommended FYB Antibody (supplied by: Log in to see )

Antigen
FYN-Binding Protein (FYB) Antibodies
  • ADAP
  • B630013F22Rik
  • fyb
  • FYB-120/130
  • p120/130
  • p120/p130
  • PRO0823
  • RGD1563421
  • SLAP-130
  • Slap130
Epitope
C-Term
29
16
9
3
2
2
2
2
Reactivity
Human, Mouse (Murine)
73
23
17
2
2
Host
Rabbit
58
11
7
Clonality
Polyclonal
Conjugate
This FYB antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
61
39
14
11
5
3
3
2
2
2
Supplier
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Catalog No. ABIN499215
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13383192 ABIN3184706 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.13383192 ABIN2155079 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13383192 ABIN1030221 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011757269 ABIN211342 IP WB Rabbit IgG AA 755-768 Log in to see Polyclonal
0.0011757269 ABIN1001738 ICC ELISA WB Rabbit IgG C-Term Log in to see Polyclonal 4
0.0011757269 ABIN799404 WB Rabbit C-Term Log in to see Polyclonal
0.0011757269 ABIN1700668 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal
0.0011757269 ABIN1711685 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal
0.0011757269 ABIN2439900 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.0011757269 ABIN1714170 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal
0.0011757269 ABIN2196226 IP WB Rabbit IgG AA 755-768 Log in to see Polyclonal
0.0011757269 ABIN1887297 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.0011757269 ABIN1583547 ICC ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-FYB antibody for Western Blotting

Similar anti-FYB Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 7 Antibodies 39 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 14 Antibodies
Immunofluorescence (IF) 1 Antibodies 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 3 Antibodies 3 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 14 Antibodies 59 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen FYN-Binding Protein (FYB) Antibodies
Epitope C-Term
(29), (16), (9), (3), (2), (2), (2), (2)
Reactivity Human, Mouse (Murine)
(73), (23), (17), (2), (2)
Host Rabbit
(58), (11), (7)
Clonality
Polyclonal
Conjugate This FYB antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(61), (39), (14), (11), (5), (3), (3), (2), (2), (2)
Pubmed 4 references available
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Product Details anti-FYB Antibody

Target Details FYB Application Details Handling References for anti-FYB Antibody (ABIN499215) Images
Specificity This antibody recognizes ADAP (C-term).
Purification Affinity chromatography purified via peptide column
Immunogen A 20 amino acid peptide from near the carboxy terminus of Human ADAP
Isotype IgG

Target Details FYB

Product Details anti-FYB Antibody Application Details Handling References for anti-FYB Antibody (ABIN499215) Images back to top
Antigen
Alternative Name FYN-Binding Protein (FYB Antibody Abstract)
Background The adhesion and degranulation adaptor protein (ADAP) was initially identified as a molecular adapter that couples T cell receptor (TCR) stimulation to the avidity of integrins governing T cell adhesion. TCR stimulation promotes the formation of a multi-protein complex containing CARMA1, MALT1, and BCL-10, which through the association of ADAP, ultimately activates the NF-kappaB family of transcription factors. More recent experiments have shown that ADAP controls optimal T cell proliferation, cytokine production, and expression of the Bcl-2 family member Bcl-x(L), suggesting that ADAP regulates T cell activation by promoting antigen-dependent T cell-antigen presenting cell (APC) activation. At least three isoforms of ADAP are known to exist.Synonyms: Adhesion and degranulation promoting adaptor protein, FYB-120/130, FYN-T-binding protein, SLAP-130, SLAP130, SLP-76-associated phosphoprotein, p120/p130
Gene ID 2533
NCBI Accession NP_955367
UniProt O15117
Pathways TCR Signaling

Application Details

Product Details anti-FYB Antibody Target Details FYB Handling References for anti-FYB Antibody (ABIN499215) Images back to top
Application Notes ELISA. Western Blot: ADAP antibody can be used for detection of ADAP at 0.5 - 1 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FYB Antibody Target Details FYB Application Details References for anti-FYB Antibody (ABIN499215) Images back to top
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-FYB Antibody (ABIN499215)

Product Details anti-FYB Antibody Target Details FYB Application Details Handling Images back to top
Background publications

Medeiros, Burbach, Mueller, Srivastava, Moon, Highfill, Peterson, Shimizu: "Regulation of NF-kappaB activation in T cells via association of the adapter proteins ADAP and CARMA1." in: Science (New York, N.Y.), Vol. 316, Issue 5825, pp. 754-8, 2007

Mueller, Thomas, Burbach, Peterson, Shimizu: "Adhesion and degranulation-promoting adapter protein (ADAP) positively regulates T cell sensitivity to antigen and T cell survival." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 6, pp. 3559-69, 2007

Rawlings, Sommer, Moreno-García: "The CARMA1 signalosome links the signalling machinery of adaptive and innate immunity in lymphocytes." in: Nature reviews. Immunology, Vol. 6, Issue 11, pp. 799-812, 2006

Griffiths, Krawczyk, Kong, Raab, Hyduk, Bouchard, Chan, Kozieradzki, Oliveira-Dos-Santos, Wakeham, Ohashi, Cybulsky, Rudd, Penninger: "Positive regulation of T cell activation and integrin adhesion by the adapter Fyb/Slap." in: Science (New York, N.Y.), Vol. 293, Issue 5538, pp. 2260-3, 2001

Images

Product Details anti-FYB Antibody Target Details FYB Application Details Handling References for anti-FYB Antibody (ABIN499215) back to top
Supplier Images
Immunofluorescence (IF) image for anti-FYB antibody (FYN-Binding Protein) (C-Term) (ABIN499215) Immunocytochemistry of ADAP in K562 cells with AP30019PU-N ADAP antibody at 10 μg/ml.
Western Blotting (WB) image for anti-FYB antibody (FYN-Binding Protein) (C-Term) (ABIN499215) Western blot analysis of ADAP in K562 cell lysate with AP30019PU-N ADAP antibody at (...