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anti-Human LAT antibody for Biochemical Assay

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Reactivity
Human
208
84
75
1
1
1
1
Host
Mouse
131
72
13
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
8
8
6
6
6
6
3
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Biochemical Assay (BCA)
157
68
45
33
29
28
19
9
6
2
2
2
2
1
1
1
Supplier
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Catalog No. ABIN2666079
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833378 ABIN2666080 BCA Mouse IgG1 Log in to see LAT1111 4

Top referenced anti-LAT antibody for Biochemical Assay

Similar anti-LAT Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 29 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 44 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Western Blotting (WB) 151 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Reactivity Human
(208), (84), (75), (1), (1), (1), (1)
Host Mouse
(131), (72), (13), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(8), (8), (6), (6), (6), (6), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Biochemical Assay (BCA)
(157), (68), (45), (33), (29), (28), (19), (9), (6), (2), (2), (2), (2), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN2666079) Images
No Cross-Reactivity Mouse (Murine)
Purification The antibody was purified by affinity chromatography.
Clone LAT1111
Isotype IgG1

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN2666079) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (linker for activation of T-cells) is a 36 kD phospho-tyrosine adaptor protein containing SH2 domains and a CVRC motif. Two LAT isoforms have been reported with approximate molecular weights of 36 kD and 38 kD. LAT is localized to lipid rafts, and is also a potential type III transmembrane protein, thought to be expressed in the juxtanuclear intracellular compartment. LAT links TCR engagement to Ras-MAPK activation, Ca2+ flux, IL-2 expression and is required for T-cell development. LAT is phosphorylated when activated by TCR cross-linking, where it recruits signaling molecules. LAT activation can be prevented by polyunsaturated fatty acids. LAT is modified by ZAP-70 and Syk phosphorylation and can also be palmitoylated. LAT has been reported to be associated with Grb2, Gads, Grap, PLC-γ1, p85 subunit PI3K, ZAP-70, Syk, Itk, Vav, SLP-76, and cbl.
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN2666079) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN2666079) Images back to top
Concentration 0.5 mg/mL
Buffer phosphate-buffered solution, pH 7.2, containing 0.09 % sodium azide. Final antibody concentration is 0.5 mg/mL.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Upon receipt, store between 2°C and 8°C.

References for anti-LAT Antibody (ABIN2666079)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Hartgroves, Lin, Langen, Zech, Weiss, Harder: "Synergistic assembly of linker for activation of T cells signaling protein complexes in T cell plasma membrane domains." in: The Journal of biological chemistry, Vol. 278, Issue 22, pp. 20389-94, 2003

Perez-Villar, Whitney, Sitnick, Dunn, Venkatesan, ODay, Schieven, Lin, Kanner: "Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav." in: Biochemistry, Vol. 41, Issue 34, pp. 10732-40, 2002

Zeyda, Staffler, Horejsi, Waldhausl, Stulnig: "LAT displacement from lipid rafts as a molecular mechanism for the inhibition of T cell signaling by polyunsaturated fatty acids." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28418-23, 2002

Zhang, Sloan-Lancaster, Kitchen, Trible, Samelson: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN2666079) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN2666079) anti-Linker For Activation of T Cells (LAT) antibody
Flow Cytometry (FACS) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN2666079) anti-Linker For Activation of T Cells (LAT) antibody (Image 2)
Western Blotting (WB) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN2666079) anti-Linker For Activation of T Cells (LAT) antibody (Image 3)