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anti-Human LAT antibody for Immunocytochemistry

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Epitope
AA 242-262, C-Term
28
19
17
15
11
9
9
7
4
4
4
4
4
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
240
104
91
2
1
1
1
1
Host
Rabbit
136
112
14
2
Clonality
Polyclonal
Conjugate
This LAT antibody is un-conjugated
9
9
8
8
8
6
5
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
193
69
59
52
49
33
22
20
16
6
2
2
2
2
1
1
Supplier
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Available images

Catalog No. ABIN3043991
$ 200.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN2624774 ICC IHC (p) WB Rabbit IgG AA 242-262 Log in to see Polyclonal
0.0009962841 ABIN1868912 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 25-221 Log in to see Polyclonal
0.0009962841 ABIN3031630 ICC IHC (p) WB Rabbit IgG C-Term Log in to see Polyclonal

Similar anti-LAT Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 53 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 20 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 49 Antibodies
Immunoprecipitation (IP) 41 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Western Blotting (WB) 172 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Epitope AA 242-262, C-Term
(28), (19), (17), (15), (11), (9), (9), (7), (4), (4), (4), (4), (4), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(240), (104), (91), (2), (1), (1), (1), (1)
Host Rabbit
(136), (112), (14), (2)
Clonality
Polyclonal
Conjugate This LAT antibody is un-conjugated
(9), (9), (8), (8), (8), (6), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(193), (69), (59), (52), (49), (33), (22), (20), (16), (6), (2), (2), (2), (2), (1), (1)
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling Images
Sequence QEAEEVEEEG APDYENLQEL N
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 1(LAT) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: linker for activation of T cells
Protein Name: Linker for activation of T-cells family member 1
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human LAT(242-262aa QEAEEVEEEGAPDYENLQELN), different from the related mouse and rat sequences by six amino acids.
Isotype IgG

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background Linker for activation of T cells, also known as LAT, is a protein which in humans is encoded by the LAT gene. This gene is mapped to 16p11.2. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor(TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.
UniProt O43561
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling Images back to top
Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for LAT is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
Preservative Thimerosal (Merthiolate), Sodium azide
Precaution of Use This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
Expiry Date 12 months

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-LAT antibody (Linker For Activation of T Cells) (AA 242-262) (ABIN3043991) Anti-LAT antibody, IHC(P) IHC(P): Human Appendicitis Tissue
Western Blotting (WB) image for anti-LAT antibody (Linker For Activation of T Cells) (AA 242-262) (ABIN3043991) Anti-LAT antibody, Western blotting All lanes: Anti LAT at 0.5ug/ml Lane 1: Human Pl...