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|Application / Reactivity||Human|
|Biochemical Assay (BCA)||2 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies|
|Flow Cytometry (FACS)||58 Antibodies|
|Functional Studies (Func)||1 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||4 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||39 Antibodies|
|Immunohistochemistry (IHC)||20 Antibodies|
|Immunohistochemistry (Formalin-fixed Sections) (IHC (f))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||47 Antibodies|
|Immunoprecipitation (IP)||29 Antibodies|
|Intracellular Staining (ICS)||6 Antibodies|
|Western Blotting (WB)||162 Antibodies|
|Antigen||Linker For Activation of T Cells (LAT) Antibodies|
|Epitope||AA 242-262, C-Term Alternatives|
|Conjugate||This LAT antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-LAT AntibodyTarget Details LAT Application Details Handling Images
|Sequence||QEAEEVEEEG APDYENLQEL N|
|Cross-Reactivity (Details)||No cross reactivity with other proteins.|
Rabbit IgG polyclonal antibody for Linker for activation of T-cells family member 1(LAT) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: linker for activation of T cells
Protein Name: Linker for activation of T-cells family member 1
|Purification||Immunogen affinity purified.|
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human LAT(242-262aa QEAEEVEEEGAPDYENLQELN), different from the related mouse and rat sequences by six amino acids.|
Target Details LATProduct Details anti-LAT Antibody Application Details Handling Images back to top
|Alternative Name||LAT (LAT Antibody Abstract)|
|Background||Linker for activation of T cells, also known as LAT, is a protein which in humans is encoded by the LAT gene. This gene is mapped to 16p11.2. The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor(TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.|
|Research Area||Signaling, Adaptive Immunity|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-LAT Antibody Target Details LAT Handling Images back to top
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for LAT is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
|Restrictions||For Research Use only|
HandlingProduct Details anti-LAT Antibody Target Details LAT Application Details Images back to top
|Reconstitution||Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.|
|Buffer||Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.|
|Preservative||Thimerosal (Merthiolate), Sodium azide|
|Precaution of Use||This product contains Thimerosal (Merthiolate),Sodium azide a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||-20 °C,4 °C|
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
|Expiry Date||12 months|
ImagesProduct Details anti-LAT Antibody Target Details LAT Application Details Handling back to top