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|Antigen||Linker For Activation of T Cells (LAT) Antibodies|
|Conjugate||This LAT antibody is conjugated to Biotin Alternatives|
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB)
|6 references available|
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Product Details anti-LAT AntibodyTarget Details LAT Application Details Handling References for anti-LAT antibody (ABIN967609) Images
1. Please refer to us for technical protocols.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
|Purification||The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.|
|Immunogen||Phosphorylated Human LAT|
Target Details LATProduct Details anti-LAT Antibody Application Details Handling References for anti-LAT antibody (ABIN967609) Images back to top
|Alternative Name||LAT (LAT Antibody Abstract)|
Engagement of the T cell receptor (TCR) induces signal transduction pathways that enhance gene transcription and cellular proliferation and differentiation. TCR ligation results in the recruitment and activation of multiple protein tyrosine kinases (PTKs), including lck, fyn, and ZAP70. Adaptor proteins, such as Grb2 and SLP-76, relay the signal to downstream effector molecules. LAT (linker for activation of T cells) is a substrate of the activated ZAP70 and functions to bridge the activated TCR and its associated PTKs with tyrosine kinase substrates. LAT is expressed as 36- and 38-kDa forms that result from post-translational modification, and as a 42-kDa form that results from alternative splicing. LAT is an integral membrane protein that is phosphorylated at five tyrosine sites upon TCR ligation. Following phosphorylation, LAT binds a number of important signaling molecules, including Grb2, Vav, PLCgamma1, and the p85 subunit of PI3K. Multiple studies have shown that functional LAT is required for T lymphocyte activation and thymocyte development.
The I58-1169 monoclonal antibody recognizes the phosphorylated tyrosine 171 (pY171) of LAT, which is one of the phosphotyrosine sites required for binding phosphoinositide 3-kinase, Grb2, and Gads.
|Molecular Weight||38 kDa|
|Research Area||Signaling, Adaptive Immunity|
|Pathways||TCR Signaling, Fc-epsilon Receptor Signaling Pathway|
Application DetailsProduct Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT antibody (ABIN967609) Images back to top
|Restrictions||For Research Use only|
HandlingProduct Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT antibody (ABIN967609) Images back to top
|Buffer||Aqueous buffered solution containing ≤0.09 % sodium azide.|
|Precaution of Use|
|Storage Comment||Store undiluted at 4°C.|
References for anti-LAT antibody (ABIN967609)Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
|Product cited in:||
Cho, Velikovsky, Swaminathan, Houtman, Samelson, Mariuzza: "Structural basis for differential recognition of tyrosine-phosphorylated sites in the linker for activation of T cells (LAT) by the adaptor Gads." in: The EMBO journal, Vol. 23, Issue 7, pp. 1441-51, 2004
Janssen, Zhang: "Adaptor proteins in lymphocyte activation." in: Current opinion in immunology, Vol. 15, Issue 3, pp. 269-76, 2003
Samelson: "Signal transduction mediated by the T cell antigen receptor: the role of adapter proteins." in: Annual review of immunology, Vol. 20, pp. 371-94, 2002
Zhu, Janssen, Zhang: "Minimal requirement of tyrosine residues of linker for activation of T cells in TCR signaling and thymocyte development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 1, pp. 325-33, 2002
Lin, Weiss: "Identification of the minimal tyrosine residues required for linker for activation of T cell function." in: The Journal of biological chemistry, Vol. 276, Issue 31, pp. 29588-95, 2001
Paz, Wang, Clarke, Lu, Stokoe, Abo: "Mapping the Zap-70 phosphorylation sites on LAT (linker for activation of T cells) required for recruitment and activation of signalling proteins in T cells." in: The Biochemical journal, Vol. 356, Issue Pt 2, pp. 461-71, 2001
ImagesProduct Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN967609) back to top