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anti-Human LAT antibody for Western Blotting

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Reactivity
Human
189
84
75
1
1
1
1
Host
Rabbit
130
54
13
2
Clonality
Polyclonal
Conjugate
This LAT antibody is un-conjugated
7
7
5
5
5
5
3
3
3
3
3
3
3
2
1
Application
ELISA, Western Blotting (WB)
137
67
33
29
27
19
10
9
6
2
2
2
2
2
1
1
Supplier
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Catalog No. ABIN1533125
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN967609 IHC (f) WB Biotin Mouse IgG1, kappa pTyr171 Log in to see I58-1169 6
0.12303223 ABIN393665 WB Mouse IgG1 kappa AA 1-234 Log in to see 3B11-1D5 5
0.12303223 ABIN967594 IHC (f) WB Mouse IgG1, kappa pTyr226 Log in to see J96-1238-58-93 5
0.12303223 ABIN1531437 ELISA WB Rabbit IgG pTyr171 Log in to see Polyclonal
0.12303223 ABIN1531336 IHC ELISA WB Rabbit IgG pTyr191 Log in to see Polyclonal
0.12303223 ABIN1532356 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN1532454 ELISA WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN3043991 ICC IHC (p) WB Rabbit IgG AA 242-262, C-Term Log in to see Polyclonal
0.12303223 ABIN2465239 ELISA WB Goat C-Term Log in to see Polyclonal
0.12303223 ABIN499024 WB Rabbit Log in to see Polyclonal
0.12303223 ABIN614546 IHC (p) IP WB Mouse IgG1 Log in to see LAT-10-17 (10-17)
0.12303223 ABIN2704956 WB Rabbit C-Term, pTyr191 Log in to see Polyclonal
0.12303223 ABIN2704957 WB Rabbit C-Term, pTyr255 Log in to see Polyclonal
0.12303223 ABIN250250 ELISA WB Goat IgG C-Term, AA 251-262 Log in to see Polyclonal
0.12303223 ABIN1876851 IHC WB Rabbit IgG Log in to see Polyclonal
0.12303223 ABIN2490633 ELISA WB Rabbit IgG Tyr161 Log in to see Polyclonal
0.12303223 ABIN2490811 ELISA WB Rabbit IgG Tyr171 Log in to see Polyclonal
0.12303223 ABIN2491155 ELISA WB Rabbit IgG Tyr191 Log in to see Polyclonal
0.12303223 ABIN1868912 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 25-221 Log in to see Polyclonal
0.12303223 ABIN3185361 ELISA WB Rabbit IgG Tyr659 Log in to see Polyclonal

Top referenced anti-LAT antibody for Western Blotting

Similar anti-LAT Antibodies

Application / Reactivity Human
Biochemical Assay (BCA) 2 Antibodies
ELISA 66 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Functional Studies (Func) 1 Antibodies
Immunochromatography (IC) 1 Antibodies
Immunocytochemistry (ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 26 Antibodies
Immunoprecipitation (IP) 25 Antibodies
Intracellular Staining (ICS) 6 Antibodies
Western Blotting (WB) 132 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Reactivity Human
(189), (84), (75), (1), (1), (1), (1)
Host Rabbit
(130), (54), (13), (2)
Clonality
Polyclonal
Conjugate This LAT antibody is un-conjugated
(7), (7), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application ELISA, Western Blotting (WB)
(137), (67), (33), (29), (27), (19), (10), (9), (6), (2), (2), (2), (2), (2), (1), (1)
Pubmed 2 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN1533125) Images
Specificity LAT Antibody detects endogenous levels of total LAT protein.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human LAT.
Isotype IgG

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN1533125) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background Molecular weight of target protein: 27 kDa
Synonyms: 36 kDa phospho-tyrosine adaptor protein, Linker for activation of T cells, p36-38, pp36
Molecular Weight 27 kDa
Gene ID 27040
OMIM 602354
UniProt O43561
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN1533125) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:10000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.632179 (NCBI Gene Symbol: LAT)

Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN1533125) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-LAT Antibody (ABIN1533125)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Zhang, Sloan-Lancaster, Kitchen, Trible, Samelson: "LAT: the ZAP-70 tyrosine kinase substrate that links T cell receptor to cellular activation." in: Cell, Vol. 92, Issue 1, pp. 83-92, 1998

Zhang, Trible, Samelson: "LAT palmitoylation: its essential role in membrane microdomain targeting and tyrosine phosphorylation during T cell activation." in: Immunity, Vol. 9, Issue 2, pp. 239-46, 1998

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN1533125) back to top
Supplier Images
Western Blotting (WB) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN1533125) Western blot analysis of extracts from Jurkat cells, using LAT (Ab-161) Antibody. The...