anti-Mouse (Murine) LAT antibody for Immunoprecipitation

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Reactivity
Mouse (Murine), Rat (Rattus)
279
118
103
3
2
1
Host
Mouse
148
139
12
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
12
12
10
8
8
7
7
7
6
6
6
5
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
210
68
66
50
40
39
22
21
13
6
3
2
2
1
1
1
1
1
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Catalog No. ABIN238425
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN94420 ICC IP WB Mouse IgG2a Log in to see LAT-1D1 5
1 ABIN4330394 IP WB Mouse IgG1 AA 31-233 Log in to see 11B-12
1 ABIN1859615 ICC IHC IP WB Rabbit IgG AA 30-237 Log in to see Polyclonal
1 ABIN4330360 ICC IF IP WB Mouse IgG2a Log in to see LAT-1D1
1 ABIN207093 ICC IP WB Mouse IgG2a Log in to see LAT-1D1
1 ABIN3200682 ICC IP WB Mouse IgG2a full length Log in to see LAT-1D1
1 ABIN1821202 IP WB Mouse IgG1, kappa AA 31-233 Log in to see
1 ABIN4330374 ICC IF IP WB PerCP Mouse IgG2a Log in to see LAT-1D1
1 ABIN4330376 ICC IF IP WB Allophycocyanin Mouse IgG2a Log in to see LAT-1D1
1 ABIN4330372 ICC IF IP WB PE Mouse IgG2a Log in to see LAT-1D1
1 ABIN3038905 IF/ICC IP WB Mouse IgG2a Log in to see

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(279), (118), (103), (3), (2), (1)
Host Mouse
(148), (139), (12), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(12), (12), (10), (8), (8), (7), (7), (7), (6), (6), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(210), (68), (66), (50), (40), (39), (22), (21), (13), (6), (3), (2), (2), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN238425) Images
Specificity The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Human
Cross-Reactivity (Details) Species reactivity (tested):Mouse and Rat.
Characteristics Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1
Purification Protein A Affinity Chromatography.
Immunogen Recombinant rat LAT.
Clone LAT-1D1
Isotype IgG2a

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT antibody (ABIN238425) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcεRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36
Gene ID 27040
UniProt O43561
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT antibody (ABIN238425) Images back to top
Application Notes Western Blotting: 5-10 μg/mLPositive control: mouse spleen. Immunoprecipitation. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT antibody (ABIN238425) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0,05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-LAT antibody (ABIN238425)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Tolar, T?mová, Dráber: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia biologica, Vol. 47, Issue 6, pp. 215-7, 2001

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT antibody (ABIN238425) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) antibody (ABIN238425) anti-Linker For Activation of T Cells (LAT) antibody
Immunohistochemistry (IHC) image for anti-Linker For Activation of T Cells (LAT) antibody (ABIN238425) anti-Linker For Activation of T Cells (LAT) antibody (Image 2)