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anti-Rat (Rattus) LAT antibody for Immunocytochemistry

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
Reactivity
Mouse (Murine), Rat (Rattus)
190
83
74
1
1
1
1
Host
Mouse
131
53
13
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
7
7
5
5
5
5
3
3
3
3
3
3
3
2
1
Application
Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
137
68
33
28
27
19
10
8
6
2
2
2
2
2
1
1
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Catalog No. ABIN94420
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010003004 ABIN207093 ICC IP WB Mouse IgG2a Log in to see LAT-1D1
0.0010003004 ABIN3200682 ICC IP WB Mouse IgG2a Log in to see LAT-1D1
0.0010003004 ABIN3038905 ICC IF IP WB Mouse IgG2a Log in to see

Top referenced anti-LAT antibody for Immunocytochemistry

Similar anti-LAT Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 19 Antibodies 23 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (IF) 2 Antibodies 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 33 Antibodies 33 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 11 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 43 Antibodies
Flow Cytometry (FACS) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(190), (83), (74), (1), (1), (1), (1)
Host Mouse
(131), (53), (13), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(7), (7), (5), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (1)
Application Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
(137), (68), (33), (28), (27), (19), (10), (8), (6), (2), (2), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN94420) Images
Specificity The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Human
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Immunogen Bacterially expressed full-length rat LAT
Clone LAT-1D1
Isotype IgG2a

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN94420) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN94420) Images back to top
Application Notes Western Blotting: Recommended dilution: 5-10 µg/mL
Positive control: mouse spleen

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN94420) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-LAT Antibody (ABIN94420)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Product cited in:

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Tolar, T?mová, Dráber: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia biologica, Vol. 47, Issue 6, pp. 215-7, 2001

Images

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Supplier Images
Immunocytochemistry (ICC) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN94420) Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets...
Immunocytochemistry (ICC) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN94420) Distribution of LAT and NTAL in separate clusters on mast cell plasma membrane sheets...