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anti-Rat (Rattus) LAT antibody for Immunoprecipitation

Recommended LAT Antibody (supplied by: Log in to see )

Antigen
Linker For Activation of T Cells (LAT) Antibodies
  • LAT
  • lat
  • LAT1
  • pp36
  • linker for activation of T cells
  • LAT
  • lat
  • Lat
Reactivity
Mouse (Murine), Rat (Rattus)
244
108
95
2
1
1
1
1
Host
Mouse
142
108
14
2
Clonality
Monoclonal
Conjugate
This LAT antibody is un-conjugated
9
9
8
8
8
6
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
2
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
186
67
51
46
39
38
22
21
14
6
2
2
2
1
1
1
Supplier
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Catalog No. ABIN238425
$ 286.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN94420 ICC IP WB Mouse IgG2a Log in to see LAT-1D1 5
1 ABIN4330360 ICC IF IP WB Mouse IgG2a Log in to see LAT-1D1
1 ABIN207093 ICC IP WB Mouse IgG2a Log in to see LAT-1D1
1 ABIN4330374 ICC IF IP WB PerCP Mouse IgG2a Log in to see LAT-1D1
1 ABIN3200682 ICC IP WB Mouse IgG2a full length Log in to see LAT-1D1
1 ABIN4330376 ICC IF IP WB Allophycocyanin Mouse IgG2a Log in to see LAT-1D1
1 ABIN4330372 ICC IF IP WB PE Mouse IgG2a Log in to see LAT-1D1
1 ABIN3038905 ICC IF IP WB Mouse IgG2a Log in to see

Top referenced anti-LAT antibody for Immunoprecipitation

Similar anti-LAT Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 19 Antibodies 22 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Immunocytochemistry (ICC) 17 Antibodies 17 Antibodies
Immunofluorescence (IF) 15 Antibodies 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 39 Antibodies 39 Antibodies
Immunohistochemistry (IHC) 2 Antibodies 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies 12 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 57 Antibodies
Flow Cytometry (FACS) 2 Antibodies

General

Antigen Linker For Activation of T Cells (LAT) Antibodies
Reactivity Mouse (Murine), Rat (Rattus)
(244), (108), (95), (2), (1), (1), (1), (1)
Host Mouse
(142), (108), (14), (2)
Clonality (Clone)
Monoclonal   ( )
Conjugate This LAT antibody is un-conjugated
(9), (9), (8), (8), (8), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1)
Application Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(186), (67), (51), (46), (39), (38), (22), (21), (14), (6), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-LAT Antibody

Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN238425) Images
Specificity The antibody LAT-1D1 reacts with mouse and rat Linker for activation of T cells (LAT), a transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.
No Cross-Reactivity Human
Cross-Reactivity (Details) Species reactivity (tested):Mouse and Rat.
Characteristics Synonyms: pp36, p36-38, 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-CellsFamily Member 1
Purification Protein A Affinity Chromatography.
Immunogen Recombinant rat LAT.
Clone LAT-1D1
Isotype IgG2a

Target Details LAT

Product Details anti-LAT Antibody Application Details Handling References for anti-LAT Antibody (ABIN238425) Images back to top
Antigen
Alternative Name LAT (LAT Antibody Abstract)
Background LAT (Linker for Activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcεRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.Synonyms: 36 kDa Phospho-Tyrosine Adapter Protein, Linker For Activation of T-Cells Family Member 1, p36-38, pp36
Gene ID 27040
UniProt O43561
Research Area Signaling, Adaptive Immunity
Pathways TCR Signaling, Fc-epsilon Receptor Signaling Pathway

Application Details

Product Details anti-LAT Antibody Target Details LAT Handling References for anti-LAT Antibody (ABIN238425) Images back to top
Application Notes Western Blotting: 5-10 μg/mLPositive control: mouse spleen. Immunoprecipitation. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-LAT Antibody Target Details LAT Application Details References for anti-LAT Antibody (ABIN238425) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0,05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-LAT Antibody (ABIN238425)

Product Details anti-LAT Antibody Target Details LAT Application Details Handling Images back to top
Background publications

Yamasaki, Ishikawa, Sakuma, Kanagawa, Cheng, Malissen, Saito: "LAT and NTAL mediate immunoglobulin E-induced sustained extracellular signal-regulated kinase activation critical for mast cell survival." in: Molecular and cellular biology, Vol. 27, Issue 12, pp. 4406-15, 2007

Lebduska, Korb, T?mová, Heneberg, Dráber: "Topography of signaling molecules as detected by electron microscopy on plasma membrane sheets isolated from non-adherent mast cells." in: Journal of immunological methods, Vol. 328, Issue 1-2, pp. 139-51, 2007

Heneberg, Lebduska, Dráberová, Korb, Dráber: "Topography of plasma membrane microdomains and its consequences for mast cell signaling." in: European journal of immunology, Vol. 36, Issue 10, pp. 2795-806, 2006

Volná, Lebduska, Dráberová, Símová, Heneberg, Boubelík, Bugajev, Malissen, Wilson, Horejsí, Malissen, Dráber: "Negative regulation of mast cell signaling and function by the adaptor LAB/NTAL." in: The Journal of experimental medicine, Vol. 200, Issue 8, pp. 1001-13, 2004

Tolar, T?mová, Dráber: "New monoclonal antibodies recognizing the adaptor protein LAT." in: Folia biologica, Vol. 47, Issue 6, pp. 215-7, 2001

Images

Product Details anti-LAT Antibody Target Details LAT Application Details Handling References for anti-LAT Antibody (ABIN238425) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN238425) anti-Linker For Activation of T Cells (LAT) antibody
Immunohistochemistry (IHC) image for anti-LAT antibody (Linker For Activation of T Cells) (ABIN238425) anti-Linker For Activation of T Cells (LAT) antibody (Image 2)