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anti-Cow (Bovine) Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Amphibian, Chicken, Cow (Bovine), Human, Monkey
797
291
244
207
120
117
103
79
78
43
40
32
31
26
26
7
5
3
3
2
2
1
1
1
1
1
1
Host
Mouse
522
268
30
12
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is conjugated to Biotin
28
25
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
667
294
289
288
240
227
218
130
104
23
15
11
8
7
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN112307
$ 445.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463598 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
0.0010463598 ABIN222824 FACS IHC (fro) IHC (p) ELISA Biotin Mouse IgG2a Log in to see
0.0010463598 ABIN3026782 FACS IF IHC WB Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365380 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365381 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365382 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365408 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
0.0010463598 ABIN4365409 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
0.0010463598 ABIN4365410 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
0.0010463598 ABIN4365413 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365414 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365415 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365416 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365417 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365418 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365419 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365420 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365421 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365423 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 Log in to see VM452
0.0010463598 ABIN4365424 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 Log in to see VM452

Top referenced anti-Vimentin antibody for Flow Cytometry

Similar anti-Vimentin Antibodies

Application / Reactivity Human Chicken Amphibian Cow (Bovine) Monkey
Western Blotting (WB) 638 Antibodies 103 Antibodies 3 Antibodies 106 Antibodies 23 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 99 Antibodies 63 Antibodies 43 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 281 Antibodies 93 Antibodies 3 Antibodies 62 Antibodies 9 Antibodies
Immunohistochemistry (IHC) 280 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Amphibian, Chicken, Cow (Bovine), Human, Monkey
(797), (291), (244), (207), (120), (117), (103), (79), (78), (43), (40), (32), (31), (26), (26), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(522), (268), (30), (12), (12), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is conjugated to Biotin
(28), (25), (21), (18), (13), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(667), (294), (289), (288), (240), (227), (218), (130), (104), (23), (15), (11), (8), (7), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 12 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112307) Images
Specificity The antibody is highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. VIM 3B4 has turned out to be the most avid mab to Vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (vimentin) of mesenchymal cells. Tumors Specifically Detected: Sarcoma (including myosarcoma), lymphoma, melanoma. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al.(1992). According to these authors, the epitope has been localized on the alpha-helical part of vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine vimentin (that could explain for the weak cross-reaction of the antibody with murine vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using human vimentin (Rogers et al., 1995).
Cross-Reactivity (Details) Species reactivity (tested):Human, Monkey, Bovine, Dog, Chicken, Amphibia (eg. Xenopus leavis).
Purification Protein A affinity chromatography
Immunogen Vimentin (purified from bovine lens).
Clone VIM 3B4
Isotype IgG2a

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112307) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112307) Images back to top
Application Notes ELISA. FACS. Cell sorting. Immunohistochemistry on frozen sections: 1: 10. Immunohistochemsitry on paraffin sections: 1: 10, 1 h at RT, protease pretreatmen t isrequired prior to antibody application.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112307) Images back to top
Format Liquid
Buffer PBS, pH 7.4
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Vimentin Antibody (ABIN112307)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Koeser, Troyanovsky, Grund, Franke: "De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components." in: Experimental cell research, Vol. 285, Issue 1, pp. 114-30, 2003

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995

Rogers, Eckelt, Nimmrich, Janssen, Schliwa, Herrmann, Franke: "Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly." in: European journal of cell biology, Vol. 66, Issue 2, pp. 136-50, 1995

Herrmann, Eckelt, Brettel, Grund, Franke: "Temperature-sensitive intermediate filament assembly. Alternative structures of Xenopus laevis vimentin in vitro and in vivo." in: Journal of molecular biology, Vol. 234, Issue 1, pp. 99-113, 1993

Bohn, Wiegers, Beuttenmüller, Traub: "Species-specific recognition patterns of monoclonal antibodies directed against vimentin." in: Experimental cell research, Vol. 201, Issue 1, pp. 1-7, 1992

Herrmann, Hofmann, Franke: "Identification of a nonapeptide motif in the vimentin head domain involved in intermediate filament assembly." in: Journal of molecular biology, Vol. 223, Issue 3, pp. 637-50, 1992

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991

Kasper, Stosiek, van Muijen, Moll: "Cell type heterogeneity of intermediate filament expression in epithelia of the human pituitary gland." in: Histochemistry, Vol. 93, Issue 1, pp. 93-103, 1990

Kasper, Karsten, Stosiek, Moll: "Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin." in: Differentiation; research in biological diversity, Vol. 40, Issue 3, pp. 207-14, 1989

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988

Jahn, Fouquet, Rohe, Franke: "Cytokeratins in certain endothelial and smooth muscle cells of two taxonomically distant vertebrate species, Xenopus laevis and man." in: Differentiation; research in biological diversity, Vol. 36, Issue 3, pp. 234-54, 1988