anti-Cow (Bovine) Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
923
401
351
220
137
131
120
82
80
52
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
570
318
30
14
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
30
28
25
23
17
16
13
13
13
13
13
13
13
13
12
10
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
735
342
326
304
253
249
248
143
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN3026782
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN112308 EIA FACS IHC (fro) IF IHC (p) WB FITC Mouse IgG2a Log in to see VIM 3B4 11
1 ABIN3026900 FACS IF IHC WB Mouse IgG1 Log in to see SPM576
1 ABIN2745279 ELISA FACS IF IHC (p) IP WB Mouse IgG1 Log in to see VM452
1 ABIN3026897 FACS IF IHC (p) Mouse IgM, kappa Log in to see LN-6
1 ABIN4365380 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see VM452
1 ABIN4365409 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
1 ABIN4365410 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
1 ABIN4365444 FACS PE Mouse IgG1 Log in to see SPM576
1 ABIN4365435 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 Log in to see SPM576
1 ABIN4365446 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 Log in to see SPM576
1 ABIN4365408 FACS ICC IF IHC (fro) IHC (p) IP WB Mouse IgG1 Log in to see SPM576
1 ABIN4365424 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 Log in to see VM452
1 ABIN4365432 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 Log in to see SPM576
1 ABIN4365434 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 647 Mouse IgG1 Log in to see SPM576
1 ABIN4365433 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 Log in to see SPM576
1 ABIN4365436 FACS Allophycocyanin Mouse IgG1 Log in to see SPM576
1 ABIN4365437 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 Log in to see SPM576
1 ABIN4365438 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 650 Mouse IgG1 Log in to see SPM576
1 ABIN4365439 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 Log in to see SPM576
1 ABIN4365440 ELISA FACS ICC IF IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 Log in to see SPM576

General

Antigen Vimentin (VIM) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
(923), (401), (351), (220), (137), (131), (120), (82), (80), (52), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(570), (318), (30), (14), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(30), (28), (25), (23), (17), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (10), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(735), (342), (326), (304), (253), (249), (248), (143), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody specifically reacts with an ~ 58 kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Purification Protein G affinity chromatography
Immunogen Recombinant protein was used as the immunogen for the Vimentin antibody.
Clone VM452
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background This antibody specifically reacts with an ~ 58 kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Gene ID 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN3026782) Flow cytometry testing of Jurkat cells and Alexa Fluor 488 conjugated Vimentin antibo...
Immunohistochemistry (IHC) image for anti-Vimentin (VIM) antibody (ABIN3026782) IHC staining of FFPE human melanoma with Vimentin antibody (VM452).
Western Blotting (WB) image for anti-Vimentin (VIM) antibody (ABIN3026782) Western blot testing of Vimentin antibody and Raji cell lysate. Predicted molecular w...