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anti-Dog (Canine) Vimentin antibody for Enzyme Immunoassay

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Amphibian, Chicken, Cow (Bovine), Dog (Canine), Human, Monkey
805
296
249
208
120
118
103
79
78
43
41
32
31
26
26
7
5
3
3
2
2
1
1
1
1
1
1
Host
Mouse
526
271
31
12
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is conjugated to FITC
27
26
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
673
300
295
294
247
228
219
132
106
23
15
11
8
7
5
3
3
3
3
1
1
1
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Catalog No. ABIN112308
$ 544.50
Plus shipping costs $45.00

Top referenced anti-Vimentin antibody for Enzyme Immunoassay

Similar anti-Vimentin Antibodies

Application / Reactivity Monkey Human
ELISA 7 Antibodies 211 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies 8 Antibodies
Flow Cytometry (FACS) 8 Antibodies 217 Antibodies
Immunochromatography (IC) 2 Antibodies 12 Antibodies
Immunocytochemistry (ICC) 7 Antibodies 241 Antibodies
Immunofluorescence (IF) 8 Antibodies
Immunohistochemistry (IHC) 12 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Western Blotting (WB) 23 Antibodies
Immunodiffusion (ID) 3 Antibodies
Immunoelectrophoresis (IEP) 3 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Amphibian, Chicken, Cow (Bovine), Dog (Canine), Human, Monkey
(805), (296), (249), (208), (120), (118), (103), (79), (78), (43), (41), (32), (31), (26), (26), (7), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(526), (271), (31), (12), (12), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is conjugated to FITC
(27), (26), (21), (18), (13), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(673), (300), (295), (294), (247), (228), (219), (132), (106), (23), (15), (11), (8), (7), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112308) Images
Specificity This antibody is highly specific for the intermediate filament protein Vimentin. Polypeptide reacting: Mr 57 000 intermediate filament protein (Vimentin) of mesenchymal cells. The binding region of monoclonal antibody VIM3B4 has been characterized by Bohn et al. (1992). According to these authors, the epitope has been localized on the alpha-helical part of Vimentin (rod domain coil 2). Due to an aa substitution at position of aa 353 in murine Vimentin (that could explain for the weak cross-reaction of the antibody with murine Vimentin) they were able to narrow down the binding region around position 353. These findings were confirmed by truncation mutagenesis experiments using Human Vimentin (Rogers et al., 1995). Clone VIM 3B4 has turned out to be the most avid mab to Vimentin. Tumors Specifically Detected: Sarcoma (including myosarcoma), lymphoma, melanoma. Tested Reactivities on Cultured Cell Lines: RD cells, glioma cells, fibroblasts (SV-80), MDCK.
Cross-Reactivity (Details) Species reactivity (tested):Human, Monkey, Bovine, Dog, Chicken, Amphibian (e.g. Xenopus leavis). Weaker Murine cross-reaction.
Purification Affinity Chromatography on Protein A
Immunogen Vimentin (purified from Bovine lens)
Clone VIM 3B4
Isotype IgG2a

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112308) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is an intermediate filament protein which is present in all cells of mesenchymal origin. Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112308) Images back to top
Application Notes ELISA. Immunoblotting. Flow Cytometry. Immunofluorescence Microscopy (5-10 μg/mL recommended)Immunohistochemistry (dilute at least 1/10 with PBS, pH 7.4). Suitable for Frozen and Paraffin-Embedded tissue and Cytological Material. Withparaffin-embedded sections, protease pretreatment is required prior to antibodyapplication. Incubation Time: 1 h at RT, extended with paraffin.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112308) Images back to top
Format Liquid
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Vimentin Antibody (ABIN112308)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Koeser, Troyanovsky, Grund, Franke: "De novo formation of desmosomes in cultured cells upon transfection of genes encoding specific desmosomal components." in: Experimental cell research, Vol. 285, Issue 1, pp. 114-30, 2003

Demirkesen, Hoede, Moll: "Epithelial markers and differentiation in adnexal neoplasms of the skin: an immunohistochemical study including individual cytokeratins." in: Journal of cutaneous pathology, Vol. 22, Issue 6, pp. 518-35, 1996

Gomi, Yokoyama, Fujimoto, Ikeda, Katoh, Itoh, Itohara: "Mice devoid of the glial fibrillary acidic protein develop normally and are susceptible to scrapie prions." in: Neuron, Vol. 14, Issue 1, pp. 29-41, 1995

Rogers, Eckelt, Nimmrich, Janssen, Schliwa, Herrmann, Franke: "Truncation mutagenesis of the non-alpha-helical carboxyterminal tail domain of vimentin reveals contributions to cellular localization but not to filament assembly." in: European journal of cell biology, Vol. 66, Issue 2, pp. 136-50, 1995

Herrmann, Eckelt, Brettel, Grund, Franke: "Temperature-sensitive intermediate filament assembly. Alternative structures of Xenopus laevis vimentin in vitro and in vivo." in: Journal of molecular biology, Vol. 234, Issue 1, pp. 99-113, 1993

Bohn, Wiegers, Beuttenmüller, Traub: "Species-specific recognition patterns of monoclonal antibodies directed against vimentin." in: Experimental cell research, Vol. 201, Issue 1, pp. 1-7, 1992

Moll, Moll: "Comparative cytokeratin analysis of sweat gland ducts and eccrine poromas." in: Archives of dermatological research, Vol. 283, Issue 5, pp. 300-9, 1991

Kasper, Stosiek, van Muijen, Moll: "Cell type heterogeneity of intermediate filament expression in epithelia of the human pituitary gland." in: Histochemistry, Vol. 93, Issue 1, pp. 93-103, 1990

Kasper, Karsten, Stosiek, Moll: "Distribution of intermediate-filament proteins in the human enamel organ: unusually complex pattern of coexpression of cytokeratin polypeptides and vimentin." in: Differentiation; research in biological diversity, Vol. 40, Issue 3, pp. 207-14, 1989

Heid, Moll, Franke: "Patterns of expression of trichocytic and epithelial cytokeratins in mammalian tissues. I. Human and bovine hair follicles." in: Differentiation; research in biological diversity, Vol. 37, Issue 2, pp. 137-57, 1988

Jahn, Fouquet, Rohe, Franke: "Cytokeratins in certain endothelial and smooth muscle cells of two taxonomically distant vertebrate species, Xenopus laevis and man." in: Differentiation; research in biological diversity, Vol. 36, Issue 3, pp. 234-54, 1988