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anti-Dog (Canine) Vimentin antibody for Immunohistochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
839
314
273
205
120
110
94
76
74
42
39
32
30
29
25
6
6
4
3
3
2
1
1
1
1
1
1
Host
Mouse
527
302
30
15
12
8
7
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
28
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
659
295
270
268
244
223
217
108
82
40
15
13
10
8
8
5
3
3
3
3
1
1
1
1
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Catalog No. ABIN3026782
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.598225 ABIN3026900 FACS IF IHC WB Mouse IgG1 Log in to see SPM576
1 ABIN152563 ICC IF IHC IHC (fro) IHC (p) WB Chicken IgY Log in to see Polyclonal 18
1 ABIN967290 IF IHC WB Mouse IgG1 Log in to see RV202 4
1 ABIN4365312 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9 1
1 ABIN4365314 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202 1
1 ABIN335272 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
1 ABIN335271 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN335380 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV202 5
1 ABIN335381 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
1 ABIN3026783 IHC Mouse IgG1 Log in to see VM452
1 ABIN4365346 FACS ICC IF IHC IHC (fro) IHC (p) WB PerCP Mouse IgG1 Log in to see RV203
1 ABIN4365313 CyTOF FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV203
1 ABIN4365462 FACS ICC IF IHC IHC (fro) IHC (p) FITC Mouse IgG1 Log in to see RV202
1 ABIN4365315 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202
1 ABIN3026784 FACS IF IHC WB Mouse IgG1 Log in to see VM452
1 ABIN3026901 FACS IF IHC WB Mouse IgG1 Log in to see SPM576

Top referenced anti-Vimentin antibody for Immunohistochemistry

Similar anti-Vimentin Antibodies

Application / Reactivity Human Chicken Dog (Canine) Pig (Porcine) Goat Cat (Feline) Cow (Bovine)
Cytometry by Time of Flight (CyTOF) 10 Antibodies 4 Antibodies 5 Antibodies 6 Antibodies 4 Antibodies 4 Antibodies 2 Antibodies
Simple Western (SimWes) 5 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 619 Antibodies 125 Antibodies 81 Antibodies 90 Antibodies 68 Antibodies 51 Antibodies 30 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Cat (Feline), Chicken, Cow (Bovine), Dog (Canine), Goat, Human, Pig (Porcine)
(839), (314), (273), (205), (120), (110), (94), (76), (74), (42), (39), (32), (30), (29), (25), (6), (6), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(527), (302), (30), (15), (12), (8), (7)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (28), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
(659), (295), (270), (268), (244), (223), (217), (108), (82), (40), (15), (13), (10), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1), (1)
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody specifically reacts with an ~ 58 kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Purification Protein G affinity chromatography
Immunogen Recombinant protein was used as the immunogen for the Vimentin antibody.
Clone VM452
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background This antibody specifically reacts with an ~ 58 kDa protein identified as Vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Vimentin antibody alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for tumor sub-classification. Expression of vimentin, when used in conjunction with keratin antibody, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with vimentin antibody. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity with vimentin antibody is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Vimentin antibody is also useful as a tissue process control reagent.
Gene ID 7431
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling Images back to top
Application Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Vimentin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin antibody (VIM) (ABIN3026782) Flow cytometry testing of Jurkat cells and Alexa Fluor 488 conjugated Vimentin antibo...
Immunohistochemistry (IHC) image for anti-Vimentin antibody (VIM) (ABIN3026782) IHC staining of FFPE human melanoma with Vimentin antibody (VM452).
Western Blotting (WB) image for anti-Vimentin antibody (VIM) (ABIN3026782) Western blot testing of Vimentin antibody and Raji cell lysate. Predicted molecular w...