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anti-Pig (Porcine) Vimentin antibody for Immunocytochemistry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Chicken
805
295
248
207
121
118
104
79
78
43
41
32
31
27
26
7
5
4
3
2
2
1
1
1
1
1
1
Host
Mouse
526
271
31
12
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
26
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
673
300
295
295
246
228
220
133
105
23
15
11
8
8
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN94492
$ 265.65
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0009962841 ABIN1580467 ICC IF IHC WB Mouse IgG1 Log in to see 2A52 2
0.0009962841 ABIN1580468 ICC IF IHC WB Mouse IgG2a Log in to see 2D1 2
0.0009962841 ABIN335272 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.0009962841 ABIN213302 ICC IHC (p) WB Mouse IgG1 pSer72 Log in to see
0.0009962841 ABIN335381 ICC FACS IHC (fro) IHC WB Mouse IgG1 Log in to see RV203 1
0.0009962841 ABIN1042494 ICC IHC (fro) FACS IHC (p) WB HRP Mouse IgG1 Log in to see RV202 5
0.0009962841 ABIN1042493 ICC IHC (fro) FACS IHC (p) WB FITC Mouse IgG1 Log in to see RV202 5
0.0009962841 ABIN1805484 ICC IHC (fro) WB HRP Mouse IgG1 Log in to see RV202
0.0009962841 ABIN1805483 ICC IHC (fro) FACS FITC Mouse IgG1 Log in to see RV202
0.0009962841 ABIN206979 ICC WB Mouse IgM Log in to see VI-01
0.0009962841 ABIN2469991 ICC IP IHC WB Mouse IgM Log in to see VI-10
0.0009962841 ABIN1724173 ICC IHC (p) IP WB Mouse IgM Log in to see VI-10
0.0009962841 ABIN269191 ICC IF IHC (p) IP IHC WB Mouse IgM Log in to see VI-10 1
0.0009962841 ABIN355074 ICC IP ELISA WB Mouse IgM Log in to see
0.0009962841 ABIN4365309 ICC IF WB Mouse IgG1 Log in to see 2A52
0.0009962841 ABIN4365310 ICC IF WB Mouse IgG1 Log in to see 2A52
0.0009962841 ABIN4365324 ICC IF WB DyLight 405 Mouse IgG1 Log in to see 2A52
0.0009962841 ABIN4365327 ICC IF IHC IHC (p) IP WB DyLight 350 Mouse IgM Log in to see VI-10
0.0009962841 ABIN4365333 ICC IF IHC IHC (p) IP WB DyLight 405 Mouse IgM Log in to see VI-10
0.0009962841 ABIN4365338 ICC IF IHC IHC (p) IP WB PE Mouse IgM Log in to see VI-10

Top referenced anti-Vimentin antibody for Immunocytochemistry

Similar anti-Vimentin Antibodies

Application / Reactivity Human Pig (Porcine) Mouse (Murine) Rat (Rattus) Chicken
Western Blotting (WB) 639 Antibodies 151 Antibodies 198 Antibodies 235 Antibodies 103 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 8 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Immunoprecipitation (IP) 100 Antibodies 67 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Human, Pig (Porcine), Mouse (Murine), Rat (Rattus), Chicken
(805), (295), (248), (207), (121), (118), (104), (79), (78), (43), (41), (32), (31), (27), (26), (7), (5), (4), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(526), (271), (31), (12), (12), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (26), (21), (18), (13), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(673), (300), (295), (295), (246), (228), (220), (133), (105), (23), (15), (11), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN94492) Images
Specificity The antibody VI-10 reacts with vimentin, a 57 kDa intermediate filament expressed in variety of mesenchymal and mesodermal cell types.
Purification Purified from ascites by precipitation methods and size-exclusion chromatography.
Purity > 95 % (by SDS-PAGE)
Clone VI-10
Isotype IgM

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN94492) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the most ubiquituos intermediate filament protein and the first to be expressed during cell differentiation. All primitive cell types express vimentin but in most non-mesenchymal cells it is replaced by other intermediate filament proteins during differentiation. Vimentin is expressed in a wide variety of mesenchymal cell types - fibroblasts, endothelial cells etc., and in a number of other cell types derived from mesoderm, e.g., mesothelium and ovarian granulosa cells. In non-vascular smooth muscle cellsand striated muscle, vimentin is often replaced by desmin, however, during regeneration, vimentin is reexpressed. Cells of the lymfo-haemopoietic system (lymphocytes, macrophages etc.) also express vimentin, sometimes in scarce amounts. Vimentin is also found in mesoderm derived epithelia, e.g. kidney (Bowman capsule), endometrium and ovary (surface epithelium), in myoepithelial cells (breast, salivary and sweat glands), an in thyroid gland epithelium. In these cell types, as in mesothelial cells, vimentin is coexpressed with cytokeratin.Furthermore, vimentin is detected in many cells from the neural crest. Particularly melanocytes express abundant vimentin. In glial cells vimentin is coexpressed with Glial Fibrillary Acidic Protein (GFAP).Vimentin is present in many different neoplasms but is particulary expressed in those originated from mesenchymal cells. Sarcomas e.g., fibrosarcoma, malignt fibrous histiocytoma, angiosarcoma, and leio- and rhabdomyosarcoma, as well as lymphomas, malignant melanoma and schwannoma, are virtually always vimentin positive. Mesoderm derived carcinomas like renal cell carcinoma, adrenal cortical carcinoma and adenocarcinomas from endometrium and ovary usually express vimentin. Also thyroid carcinomas are vimentin positive. Any low differentiated carcinoma may express some vimentin.Vimentin is frequently included in the so-called primary panel (together with CD45, cytokeratin, and S-100 protein). Intense staining reaction for vimentin without coexpression of other intermediate filament proteins is strongly suggestive of a mesenchymal tumour or malignant melanoma.
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN94492) Images back to top
Application Notes Immunohistochemistry (paraffin sections) Recommended dilution: 5 µg/mL
Positive tissue: skin fibroblastImmunocytochemistry
Staining technique: RBL basophilic leukemia cell line: (a) Fix cells for 10 min in methanol at -20 °C and for 6 min in acetone at -20 °C, (b) Fix cells directly in methanol for 10 min at -20 °C or in acetone for 10 min at -20 °C.
Incubation: 45 min RT
Positive control: 3T3 mouse Swiss albino fibroblast cell lineRBL rat basophilic cell line

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN94492) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-Vimentin Antibody (ABIN94492)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Dráberová, Zíková, Dráber: "Monoclonal antibody VI-10 specific for vimentin." in: Folia biologica, Vol. 45, Issue 1, pp. 35-6, 2000

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN94492) back to top
Supplier Images
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN94492) Immunofluorescence staining (chicken erythrocytes)
Immunofluorescence (IF) image for anti-Vimentin antibody (VIM) (ABIN94492) Immunofluorescence staining (rat basophils)
Immunoprecipitation (IP) image for anti-Vimentin antibody (VIM) (ABIN94492) Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10