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|Application / Reactivity||Sheep (Ovine)||Rat (Rattus)|
|Flow Cytometry (FACS)||4 Antibodies||17 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||35 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||52 Antibodies|
|Immunohistochemistry (IHC)||1 Antibodies||63 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||20 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||4 Antibodies||46 Antibodies|
|Western Blotting (WB)||2 Antibodies||133 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||25 Antibodies|
|Immunoprecipitation (IP)||16 Antibodies|
|Antigen||Vimentin (VIM) Antibodies|
|Reactivity||Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine) Alternatives|
|Conjugate||This Vimentin antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
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Product Details anti-Vimentin AntibodyTarget Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images
|Specificity||The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.|
|Immunogen||V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.|
Target Details VimentinProduct Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
|Alternative Name||Vimentin (VIM Antibody Abstract)|
|Background||Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.Synonyms: VIM|
|Research Area||Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers|
|Pathways||TCR Signaling, Caspase Cascade in Apoptosis|
Application DetailsProduct Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
This antibody is suitable for Immunoblotting, Immunocytochemistry,Immunohistochemistry on frozen sections and paraffin-embedded tissues and Flowcytometry. Recommended dilutions: Flow cytometry: 1/25-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Immunoblotting applications: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112184) Images back to top
|Buffer||PBS with 0.09 % Sodium Azide as preservative|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing. Do not freeze working dilutions.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the original vial at 2-8 °C. After reconstitution, aliquot and store stock solution or aliquots thereof at -20 °C.|
References for anti-Vimentin Antibody (ABIN112184)Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Corver, Koopman, van der Aa et al.: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000 (PubMed).
Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984 (PubMed).
Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987 (PubMed).
Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987 (PubMed).
Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985 (PubMed).