anti-Sheep (Ovine) Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
928
405
356
220
138
131
120
83
81
51
51
43
41
37
30
12
8
7
5
5
4
3
3
2
2
1
1
1
1
1
Host
Mouse
569
321
30
17
12
9
9
6
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
739
344
327
304
255
251
249
143
87
40
15
14
13
9
8
7
5
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN4365312
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN112184 FACS IHC (fro) IF IHC (p) WB Mouse IgG1 Log in to see V9 5
1 ABIN959513 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see V9
1 ABIN3026897 FACS IF IHC (p) Mouse IgM, kappa Log in to see LN-6
1 ABIN2964611 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 full length Log in to see V9
1 ABIN4365454 FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365456 FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365455 FACS IF IHC (p) Mouse IgM Log in to see LN-6
1 ABIN4365339 FACS PE Mouse IgG1 Log in to see V9
1 ABIN4365334 FACS ICC IF IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see V9
1 ABIN4365375 FACS ICC IF IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see V9
1 ABIN4365469 FACS IHC (p) Alexa Fluor 405 Mouse IgM Log in to see LN-6
1 ABIN4365476 FACS IHC (p) FITC Mouse IgM Log in to see LN-6
1 ABIN4365480 FACS IHC (p) DyLight 755 Mouse IgM Log in to see LN-6
1 ABIN4365471 FACS IHC (p) Alexa Fluor 647 Mouse IgM Log in to see LN-6
1 ABIN4365472 FACS IHC (p) Alexa Fluor 700 Mouse IgM Log in to see LN-6
1 ABIN4365405 FACS ICC IF IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see V9
1 ABIN4365367 FACS ICC IF IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see V9
1 ABIN4365470 FACS IHC (p) Alexa Fluor 488 Mouse IgM Log in to see LN-6
1 ABIN4365475 FACS IHC (p) DyLight 650 Mouse IgM Log in to see LN-6
1 ABIN4365477 FACS IHC (p) DyLight 680 Mouse IgM Log in to see LN-6

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Dog (Canine), Human, Pig (Porcine), Rat (Rattus), Sheep (Ovine)
(928), (405), (356), (220), (138), (131), (120), (83), (81), (51), (51), (43), (41), (37), (30), (12), (8), (7), (5), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(569), (321), (30), (17), (12), (9), (9), (6)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(739), (344), (327), (304), (255), (251), (249), (143), (87), (40), (15), (14), (13), (9), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images
Specificity V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Protein A or G purified
Immunogen Clone V9 is a mouse monoclonal IgG1 antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with vimentin isolated from porcine lens.
Clone V9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Gene Symbol: VIM
Gene ID 7431
Research Area Lineage Markers, Cell/Tissue Markers, Cytoskeleton, Neural Stem Cell marker
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN4365312) Images back to top
Application Notes Western Blot 1:100-1:1000, Flow Cytometry 1:25-1:200, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 1:10-1:500, Immunohistochemistry-Paraffin 1:25-1:200, Immunohistochemistry-Frozen 1:25-1:200, CyTOF-readyFor staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for Flow Cytometry, and for Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN4365312) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-Vimentin antibody (ABIN4365312)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Product cited in:

Rigoutsos, Lee, Nam, Anfossi, Pasculli, Pichler, Jing, Rodriguez-Aguayo, Telonis, Rossi, Ivan, Catela Ivkovic, Fabris, Clark, Ling, Shimizu, Redis, Shah, Zhang, Okugawa, Jung, Tsirigos, Huang, Ferdin et al.: "N-BLR, a primate-specific non-coding transcript leads to colorectal cancer invasion and migration. ..." in: Genome biology, Vol. 18, Issue 1, pp. 98, 2017

Hu, Ling, Xu, Han: "Fibroblast-like cells differentiated from adipose-derived mesenchymal stem cells for vocal fold wound healing." in: PLoS ONE, Vol. 9, Issue 3, pp. e92676, 2014 (Sample species: Dog (Canine)). Further details: Immunocytochemistry,Immunofluorescence

Images

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN4365312) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - Analysis of PE conjugate of NBP1-97670. An ...
Flow Cytometry (FACS) image for anti-Vimentin (VIM) antibody (ABIN4365312) Flow Cytometry: Vimentin Antibody (V9) - An intracellular stain was performed on Jur...