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anti-Sheep (Ovine) Vimentin antibody for Flow Cytometry

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • cb28
  • CTRCT30
  • VIM
  • vim
  • vim1
  • vim2
  • vime
  • Vimentin
Reactivity
Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine)
797
290
244
207
121
117
103
79
78
42
40
32
31
27
26
7
5
4
2
2
2
1
1
1
1
1
1
Host
Mouse
522
268
30
12
12
7
5
Clonality
Monoclonal
Conjugate
This Vimentin antibody is un-conjugated
28
26
21
18
13
13
11
11
11
11
11
11
11
11
11
11
9
5
5
5
2
2
2
2
2
2
2
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
666
294
289
287
240
227
218
130
104
23
15
11
8
8
5
3
3
3
3
1
1
1
Supplier
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Catalog No. ABIN112184
€ 371.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010463598 ABIN4365454 FACS IF IHC (p) Mouse IgM Log in to see LN-6
0.0010463598 ABIN4365455 FACS IF IHC (p) Mouse IgM Log in to see LN-6
0.0010463598 ABIN4365456 FACS IF IHC (p) Mouse IgM Log in to see LN-6
0.0010463598 ABIN2964611 ICC IHC (fro) FACS IHC (p) WB Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365312 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365329 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 350 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365334 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 405 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365339 FACS ICC IF IHC IHC (fro) IHC (p) WB PE Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365345 FACS PerCP Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365351 FACS ICC IF IHC IHC (fro) IHC (p) WB Allophycocyanin Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365357 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 755 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365367 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 488 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365373 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 405LS Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365375 FACS ICC IF IHC IHC (fro) IHC (p) WB DyLight 680 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365387 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 405 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365393 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 488 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365399 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 647 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365405 FACS ICC IF IHC IHC (fro) IHC (p) WB Alexa Fluor 700 Mouse IgG1 Log in to see V9
0.0010463598 ABIN4365469 FACS IHC (p) Alexa Fluor 405 Mouse IgM Log in to see LN-6
0.0010463598 ABIN4365470 FACS IHC (p) Alexa Fluor 488 Mouse IgM Log in to see LN-6

Top referenced anti-Vimentin antibody for Flow Cytometry

Similar anti-Vimentin Antibodies

Application / Reactivity Sheep (Ovine) Rat (Rattus)
Flow Cytometry (FACS) 41 Antibodies 71 Antibodies
Immunocytochemistry (ICC) 14 Antibodies 131 Antibodies
Immunofluorescence (IF) 21 Antibodies 153 Antibodies
Immunohistochemistry (IHC) 14 Antibodies 110 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 15 Antibodies 51 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 41 Antibodies 117 Antibodies
Western Blotting (WB) 15 Antibodies 233 Antibodies
ELISA 33 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunoprecipitation (IP) 31 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Human, Potoroo, Rat (Rattus), Sheep (Ovine)
(797), (290), (244), (207), (121), (117), (103), (79), (78), (42), (40), (32), (31), (27), (26), (7), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(522), (268), (30), (12), (12), (7), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This Vimentin antibody is un-conjugated
(28), (26), (21), (18), (13), (13), (11), (11), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(666), (294), (289), (287), (240), (227), (218), (130), (104), (23), (15), (11), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images
Specificity The antibody clone V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
Purification Purified
Immunogen V9 is an antibody derived by fusion of PAI mouse myeloma cells with spleen cells from a BALB/c mouse immunized with Vimentin isolated from Porcine lens.
Clone V9
Isotype IgG1

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN112184) Images back to top
Application Notes This antibody is suitable for Immunoblotting, Immunocytochemistry,Immunohistochemistry on frozen sections and paraffin-embedded tissues and Flowcytometry. Recommended dilutions: Flow cytometry: 1/25-1/200. Immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) asdetection reagent: 1/25-1/200. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citrate buffer ( pH 6.0) is required. Immunoblotting applications: 1/100-1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN112184) Images back to top
Concentration 1.0 mg/mL
Buffer PBS with 0.09 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing. Do not freeze working dilutions.
Storage 4 °C/-20 °C
Storage Comment Store the original vial at 2-8 °C. After reconstitution, aliquot and store stock solution or aliquots thereof at -20 °C.

References for anti-Vimentin Antibody (ABIN112184)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Corver, Koopman, van der Aa, Regensburg, Fleuren, Cornelisse: "Four-color multiparameter DNA flow cytometric method to study phenotypic intratumor heterogeneity in cervical cancer." in: Cytometry, Vol. 39, Issue 2, pp. 96-107, 2000

Van Muijen, Ruiter, Warnaar: "Coexpression of intermediate filament polypeptides in human fetal and adult tissues." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 57, Issue 4, pp. 359-69, 1987

Van Muijen, Warnaar, Ponec: "Differentiation-related changes of cytokeratin expression in cultured keratinocytes and in fetal, newborn, and adult epidermis." in: Experimental cell research, Vol. 171, Issue 2, pp. 331-45, 1987

Tölle, Weber, Osborn: "Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple keratins allow insight into the composition of particular 10 nm filaments." in: European journal of cell biology, Vol. 38, Issue 2, pp. 234-44, 1985

Osborn, Debus, Weber: "Monoclonal antibodies specific for vimentin." in: European journal of cell biology, Vol. 34, Issue 1, pp. 137-43, 1984