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anti-Xenopus laevis Vimentin antibody for Immunohistochemistry (Frozen Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Chicken, Cow (Bovine), Hamster, Human, Mouse (Murine), Xenopus laevis
857
371
316
221
138
131
115
84
79
50
49
42
41
35
30
10
7
6
5
5
4
3
3
2
2
2
1
1
1
1
1
1
Host
Guinea Pig
530
302
30
15
12
8
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
28
28
23
20
15
14
12
11
11
11
11
11
11
11
11
9
5
5
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
683
301
282
267
249
221
217
107
84
40
15
14
13
10
8
8
5
3
3
3
3
1
1
1
1
1
Supplier
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Catalog No. ABIN126094
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365315 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202
1 ABIN225110 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see

Top referenced anti-Vimentin antibody for Immunohistochemistry (Frozen Sections)

Similar anti-Vimentin Antibodies

Application / Reactivity Human Mouse (Murine) Chicken Cow (Bovine) Hamster Xenopus laevis
Immunofluorescence (fixed cells) (IF/ICC) 13 Antibodies 8 Antibodies 2 Antibodies 1 Antibodies
Cytometry by Time of Flight (CyTOF) 10 Antibodies 4 Antibodies 2 Antibodies 3 Antibodies 2 Antibodies
Simple Western (SimWes) 5 Antibodies 5 Antibodies 2 Antibodies
Western Blotting (WB) 653 Antibodies 238 Antibodies 99 Antibodies 104 Antibodies 30 Antibodies 7 Antibodies
Transfected Cell Culture (TCC) 1 Antibodies

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Cow (Bovine), Hamster, Human, Mouse (Murine), Xenopus laevis
(857), (371), (316), (221), (138), (131), (115), (84), (79), (50), (49), (42), (41), (35), (30), (10), (7), (6), (5), (5), (4), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Guinea Pig
(530), (302), (30), (15), (12), (8), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(28), (28), (23), (20), (15), (14), (12), (11), (11), (11), (11), (11), (11), (11), (11), (9), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(683), (301), (282), (267), (249), (221), (217), (107), (84), (40), (15), (14), (13), (10), (8), (8), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin Antibody (ABIN126094) Images
Specificity Specific detection of Vimentin (Mr 57,000 polypeptide). Tumors specifically detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
Cross-Reactivity (Details) Species reactivity (expected):Hamster, Chicken, Xenopus.
Species reactivity (tested):Human, Bovine, Mouse.
Purification Serum
Immunogen Vimentin purified from calf lens

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin Antibody (ABIN126094) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways TCR Signaling, Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin Antibody (ABIN126094) Images back to top
Application Notes Immunohistochemistry on frozen sections: 1/100. Immunohistochemistry on paraffin sections: 1/50, 1h at RT. Immunoblotting: 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin Antibody (ABIN126094) Images back to top
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Vimentin Antibody (ABIN126094)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Boehnke, Mirancea, Pavesio, Fusenig, Boukamp, Stark: "Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents." in: European journal of cell biology, Vol. 86, Issue 11-12, pp. 731-46, 2007

Magin, Bader, Freudenmann, Franke: "De novo formation of cytokeratin filaments in calf lens cells and cytoplasts after transfection with cDNAs or microinjection with mRNAs encoding human cytokeratins." in: European journal of cell biology, Vol. 53, Issue 2, pp. 333-48, 1991