anti-Xenopus laevis Vimentin antibody for Immunohistochemistry (Frozen Sections)

Recommended Vimentin Antibody (supplied by: Log in to see )

Antigen
Vimentin (VIM) Antibodies
  • vime
  • CTRCT30
  • cb28
  • vim
  • vim1
  • vim2
  • VIM
  • Vimentin
  • vimentin
  • Vimentin
  • vimentin-like
  • VIM
  • vim
  • vime
  • Vim
  • vim-a
  • LOC100522394
Reactivity
Chicken, Cow (Bovine), Hamster, Human, Mouse (Murine), Xenopus laevis
928
406
355
221
137
131
121
83
81
52
51
43
41
36
30
12
8
7
5
4
4
3
3
2
2
1
1
1
1
1
Host
Guinea Pig
570
321
30
17
12
9
8
6
Clonality
Polyclonal
Conjugate
This Vimentin antibody is un-conjugated
29
27
26
23
18
16
13
13
13
13
13
13
13
13
12
11
7
7
5
3
3
3
3
3
3
3
3
3
3
2
2
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
739
344
328
305
255
252
250
143
87
40
15
14
13
10
8
7
5
3
3
3
1
1
1
1
1
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Catalog No. ABIN126094
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4365315 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 Log in to see RV202
1 ABIN225110 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see

General

Antigen Vimentin (VIM) Antibodies
Reactivity Chicken, Cow (Bovine), Hamster, Human, Mouse (Murine), Xenopus laevis
(928), (406), (355), (221), (137), (131), (121), (83), (81), (52), (51), (43), (41), (36), (30), (12), (8), (7), (5), (4), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Guinea Pig
(570), (321), (30), (17), (12), (9), (8), (6)
Clonality
Polyclonal
Conjugate This Vimentin antibody is un-conjugated
(29), (27), (26), (23), (18), (16), (13), (13), (13), (13), (13), (13), (13), (13), (12), (11), (7), (7), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(739), (344), (328), (305), (255), (252), (250), (143), (87), (40), (15), (14), (13), (10), (8), (7), (5), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-Vimentin Antibody

Target Details Vimentin Application Details Handling References for anti-Vimentin antibody (ABIN126094) Images
Specificity Specific detection of Vimentin (Mr 57,000 polypeptide). Tumors specifically detected: Sarcoma (including myosarcoma), lymphoma, melanoma.
Cross-Reactivity (Details) Species reactivity (expected):Hamster, Chicken, Xenopus.
Species reactivity (tested):Human, Bovine, Mouse.
Purification Serum
Immunogen Vimentin purified from calf lens

Target Details Vimentin

Product Details anti-Vimentin Antibody Application Details Handling References for anti-Vimentin antibody (ABIN126094) Images back to top
Antigen
Alternative Name Vimentin (VIM Antibody Abstract)
Background Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain-dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared with carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. Vimentin antibodies are thus of value in the differential diagnosis of undifferentiated neoplasms and malignant tumors. They are generally used with a panel of other antibodies including those recognizing cytokeratins, lymphoid markers, S100, desmin and neurofilaments.Synonyms: VIM
Gene ID 7431
NCBI Accession NP_003371
UniProt P08670
Research Area Lineage Markers, Cytoskeleton, Neural Stem Cell marker, Cell/Tissue Markers
Pathways Caspase Cascade in Apoptosis

Application Details

Product Details anti-Vimentin Antibody Target Details Vimentin Handling References for anti-Vimentin antibody (ABIN126094) Images back to top
Application Notes Immunohistochemistry on frozen sections: 1/100. Immunohistochemistry on paraffin sections: 1/50, 1h at RT. Immunoblotting: 1/2000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details References for anti-Vimentin antibody (ABIN126094) Images back to top
Format Liquid
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References for anti-Vimentin antibody (ABIN126094)

Product Details anti-Vimentin Antibody Target Details Vimentin Application Details Handling Images back to top
Background publications

Boehnke, Mirancea, Pavesio, Fusenig, Boukamp, Stark: "Effects of fibroblasts and microenvironment on epidermal regeneration and tissue function in long-term skin equivalents." in: European journal of cell biology, Vol. 86, Issue 11-12, pp. 731-46, 2007

Magin, Bader, Freudenmann, Franke: "De novo formation of cytokeratin filaments in calf lens cells and cytoplasts after transfection with cDNAs or microinjection with mRNAs encoding human cytokeratins." in: European journal of cell biology, Vol. 53, Issue 2, pp. 333-48, 1991