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anti-Mouse (Murine) Adenomatous Polyposis Coli antibody for Immunohistochemistry (Frozen Sections)

Recommended Adenomatous Polyposis Coli Antibody (supplied by: Log in to see )

Antigen
Adenomatous Polyposis Coli (APC) Antibodies
  • AI047805
  • APC1
  • AU020952
  • AW124434
  • BTPS2
  • CC1
  • dp2
  • DP2
  • DP2.5
  • dp2.5
  • DP3
  • dp3
  • fap
  • fpc
  • GS
  • mAPC
  • Min
  • PPP1R46
  • RATAPC
  • xapc
Epitope
AA 2636-2845
24
22
17
15
13
10
4
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
115
72
42
6
4
2
2
1
1
Host
Rabbit
123
15
14
2
Clonality
Polyclonal
Conjugate
Un-conjugated
7
7
4
2
2
2
2
2
2
2
2
2
2
2
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
93
60
41
29
26
22
13
8
4
4
2
1
1
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Catalog No. ABIN1858038
$ 396.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00088051456 ABIN184534 ICC IHC (fro) IF IP WB Mouse IgG1 Log in to see c-APC 28-9

Similar anti-Adenomatous Polyposis Coli Antibodies

Application / Reactivity Mouse (Murine)
ELISA 33 Antibodies
Enzyme Immunoassay (EIA) 3 Antibodies
FLISA 1 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 52 Antibodies

General

Antigen Adenomatous Polyposis Coli (APC) Antibodies
Epitope AA 2636-2845
(24), (22), (17), (15), (13), (10), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(115), (72), (42), (6), (4), (2), (2), (1), (1)
Host Rabbit
(123), (15), (14), (2)
Clonality
Polyclonal
Conjugate Un-conjugated
(7), (7), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(93), (60), (41), (29), (26), (22), (13), (8), (4), (4), (2), (1), (1)
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Product details

Target details Application Details Handling Images
Sequence MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SGAES KPLIYQMAPP VSKTEDVWVR IEDCPINNPR SGRSPTGNTP PVIDSVSEKG SSSIKDSKDS KDTHGKQSVG SGSPVQTVGL ETRLNSFVQV EAPEQKGTEA KPGQSNPVSI AETAETCIAE RTPFSSSSSS KHSSPSGTVA ARVTPFNYNP SPRKSSADST SARPSQIPTP VSTNTKKRDS KTDITESSGA QSPKRHSGSY LVTSV
Specificity It has been selected for its ability to recognize APC in immunohistochemical staining and Western blotting.
Purification Affinity Chromatography
Immunogen APC (AA 2636-2845)
Isotype IgG

Target details

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Antigen
Alternative Name Adenomatosis Polyposis Coli Protein (APC) (APC Antibody Abstract)
Pathways WNT Signaling

Application Details

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Application Notes Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
Comment

Content: The quality control contains recombinant APC (Ser2636~Val2845) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2% SDS, 200 mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.

Restrictions For Research Use only

Handling

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Format Liquid
Concentration 200 µg/mL
Buffer Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze/thaw cycles
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

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Supplier Images
Western Blotting (WB) image for anti-Adenomatous Polyposis Coli (APC) (AA 2636-2845) antibody (ABIN1858038) anti-Adenomatous Polyposis Coli (APC) (AA 2636-2845) antibody