|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||12 Antibodies||33 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||3 Antibodies|
|Immunochromatography (IC)||1 Antibodies|
|Immunocytochemistry (ICC)||21 Antibodies||23 Antibodies|
|Immunofluorescence (IF)||22 Antibodies||32 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||32 Antibodies||31 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||28 Antibodies||29 Antibodies|
|Immunoprecipitation (IP)||5 Antibodies|
|Western Blotting (WB)||39 Antibodies|
|Antigen||Adenomatous Polyposis Coli (APC) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|Supplier||Log in to see|
Product detailsTarget details Application Details Handling Images
|Specificity||This antibody detects endogenous levels of APC / DP2.5 protein (region surrounding Gln2829).|
Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
|Purification||The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen|
|Purity||> 95 % pure by SDS-PAGE|
Target detailsProduct details Application Details Handling Images back to top
|Alternative Name||APC / DP2.5 (APC Antibody Abstract)|
|Background||Adenomatous polyposis coli (APC) is a protein that plays a critical role in several cellular processes. APC is a tumor suppressor, regulates cell division, helps ensure that the number of chromosomes in a cell is correct following cell division and associates with other proteins involved in cell attachment and signaling. APC regulates ß-catenin levels through Wnt-signaling and is involved in actin cytoskeletal integrity, cell-cell adhesion and cell migration. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product.Synonyms: Adenomatous polyposis coli protein, Deleted in polyposis 2.5|
|Molecular Weight||approx. 310 kDa|
Application DetailsProduct details Target details Handling Images back to top
ELISA: 1: 40000approx. 1: 60000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct details Target details Application Details Images back to top
|Buffer||Phosphate buffered saline (PBS), pH 7.2, 0.05 % Sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ImagesProduct details Target details Application Details Handling back to top