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anti-TBP-Associated Factor 172 (BTAF1) Antibodies

BTAF1 encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. Additionally we are shipping and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
BTAF1 9044 O14981
BTAF1    
BTAF1    
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Top anti-TBP-Associated Factor 172 Antibodies at antibodies-online.com

Showing 10 out of 29 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Human Rabbit Un-conjugated IP, WB WB Image Sample(30μg whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: Hep G2 , F: MOLT4 , G: Raji , 5% SDS PAGE antibody diluted at 1:1000 IP Image TAF172 antibody immunoprecipitates TAF172 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TAF172 protein by 2 μg of TAF172 antibody , 5% SDS-PAGE The immunoprecipitated TAF172 protein was detected by TAF172 antibody , diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent. 100 μL Log in to see 3 to 4 Days
$466.18
Details
Human Rabbit Un-conjugated ChIP, IP, WB Western blot analysis of TAF172 expression in Hela (A), MCF7 (B), rat testis (C) whole cell lysates. 200 μL Log in to see 13 to 14 Days
$487.50
Details
Human Rabbit Un-conjugated IP, WB Western Blot and Immunoprecipitation: BTAF1 Antibody [ABIN252839] - Detection of Human BTAF1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti- BTAF1 antibody ABIN252839 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. BTAF1 was immunoprecipitated more efficiently by rabbit anti-BTAF1 antibodies ABIN252840 and ABIN252841, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B). 100 μL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated IP Western Blot: BTAF1 Antibody [ABIN252840] - Detection of Human BTAF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody ABIN252840 used for IP at 3 mcg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies ABIN252839 and ABIN252841, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B). 100 μL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated WB Western Blot and Immunoprecipitation: BTAF1 Antibody [ABIN252842] - Detection of Human BTAF1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB, 1 mg for IP, 20% of IP loaded) and 293T (T, 50 mcg) cells. Antibodies: Affinity purified rabbit anti- BTAF1 antibody ABIN252842 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. BTAF1 was immunoprecipitated more efficiently by rabbit anti-BTAF1 antibodies ABIN252839, ABIN252840, and ABIN252841, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B). 100 μL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated IP Western Blot: BTAF1 Antibody [ABIN252841] - Detection of Human BTAF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BTAF1 antibody ABIN252841 used for IP at 3 mcg/mg lysate (A and B). BTAF1 was also immunoprecipitated by rabbit anti-BTAF1 antibodies ABIN252839 and ABIN252840, which recognize other epitopes. Detection: Chemiluminescence with exposure times of 3 seconds (A) and 30 seconds (B). 100 μL Log in to see 7 to 9 Days
$556.31
Details
Human Rabbit Un-conjugated ELISA, WB   100 μL Log in to see 16 Days
$298.18
Details
Human Rabbit Un-conjugated ChIP, IHC, IP, WB Western blot analysis of extracts of various cell lines, using BTAF1 antibody (ABIN5973427) at 1/1000 dilution. Immunoprecipitation analysis of 150 μg extracts of HeLa cells using 3 μg BTAF1 antibody (ABIN5973427). Western blot was performed from the immunoprecipitate using BTAF1 antibody (ABIN5973427) at a dilition of 1/500. 100 μL Log in to see 11 to 16 Days
$426.40
Details
Human Rabbit Un-conjugated IP, WB Western blot analysis of extracts of various cell lines, using BTAF1 antibody. Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody. 120 μL Log in to see 12 to 14 Days
$251.90
Details
Human Mouse Un-conjugated DB, ICC, FACS, IP, WB HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-BTAF1 antibody (shaded) or isotype control (unshaded) followed by Alexa Fluor 488 conjugated goat anti-mouse IgG. Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were stained with anti-BTAF1 antibody. 50 μg Log in to see 6 to 8 Days
$285.71
Details

More Antibodies against TBP-Associated Factor 172 Interaction Partners

Human TBP-Associated Factor 172 (BTAF1) interaction partners

  1. Results support a model for a BTAF1-mediated release of TBP (show TBP Antibodies)-NC2 (show GTF2H5 Antibodies) complexes from chromatin.

  2. study with the first structural model of the B-TFIID complex and map of its key functional domains

  3. physical cooperation between BTAF1 and NC2alpha in TBP (show TBP Antibodies) regulation

  4. We propose that these density rearrangements in the structure are a likely reflection of the plasticity of the interactions between TFIID and its many partner proteins.

  5. histone acetylation, although it occurs, is dispensable for TFIID (show TBP Antibodies) and SWI (show SMARCA1 Antibodies)/SNF (show SNRPA Antibodies) recruitment by the strong enhancers

TBP-Associated Factor 172 (BTAF1) Antigen Profile

Protein Summary

This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters.

Gene names and symbols associated with BTAF1

  • B-TFIID TATA-box binding protein associated factor 1 (BTAF1) antibody
  • TBP-associated factor 172 (BACOIKO008_36) antibody
  • MOT1 antibody
  • TAF(II)170 antibody
  • TAF172 antibody
  • TAFII170 antibody

Protein level used designations for BTAF1

ATP-dependent helicase BTAF1 , B-TFIID transcription factor-associated 170 kDa subunit , BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae) , Mot1 homolog , TATA-binding protein-associated factor 172 , TBP-associated factor 172

GENE ID SPECIES
9044 Homo sapiens
6166901 Oikopleura dioica
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