Loading Control Detection Set Beta-Actin, GAPDH
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- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB)
- Specificity
- The Beta-Actin and GAPDH antibodies are human, mouse, and rat reactive. These antibodies are expected to also recognize chimpanzee, cow, dog, chicken, and zebrafish proteins. The Beta-Actin antibody is also expected to recognize Xenopus and Drosophila proteins.
- Purification
- Antibodies are supplied as affinity chromatography purified IgG.
- Components
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Beta-Actin Antibody (CT)
GAPDH Antibody (CT),
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- Application Notes
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These polyclonal antibodies can be used for detection of Beta-Actin and GAPDH by immunoblot at 1-2 μg/mL.
Optimal dilutions for each application to be determined by the researcher. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Stable at 4 °C for three months, store at -20 °C for up to one year.
- Expiry Date
- 12 months
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- Background
- Loading controls are used in immunoblots to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample. This ensures constant expression levels. Thus housekeeping genes such as Beta-actin, Alpha-tubulin, and GAPDH are frequently chosen for use as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Globular tubulin subunits made up of alpha- and beta-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism.
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