Swine-Origin and Seasonal Flu H1N1 Protein Detection Set

Catalog No. ABIN2456021

Product Details

Reactivity: Influenza A Virus H1N1

Host: Rabbit

Clonality: Polyclonal

Application: ELISA

Characteristics: Concentrations: Antibody 1 mg/mL, Peptide 200 μg/mL

Purification: Antibodies are supplied as affinity chromatography purified IgG.

Components: Seasonal H1N1 Hemagglutinin Antibody, (50 μg)
Seasonal H1N1 Hemagglutinin Antibody, (50 μg)
Swine-Origin H1N1 Hemagglutinin Antibody, (50 μg)
Swine-Origin H1N1 Hemagglutinin Antibody, (50 μg)
Seasonal H1N1 Neuraminidase Antibody, (50 μg)
Swine-Origin H1N1 Neuraminidase Antibody, (50 μg)
Seasonal H1N1 Hemagglutinin Peptide, P
Seasonal H1N1 Hemagglutinin Peptide, P
Swine-Origin H1N1 Hemagglutinin Peptide, P
Swine-Origin H1N1 Hemagglutinin Peptide, P
Seasonal H1N1 Neuraminidase Peptide, P
Swine-Origin H1N1 Neuraminidase Peptide, P

Application Details

Application Notes: These polyclonal antibodies can be used for detection of H1N1 Hemagglutinin or Neuraminidase proteins in bodily fluid or tissue by ELISA. Immunogenic peptides are provided as positive controls and to determine protein concentration. Each antibody will detect 2 ng of its corresponding peptide.
Optimal dilutions for each application to be determined by the researcher.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Stable at 4 °C for three months, store at -20 °C for up to one year.

Expiry Date: 12 months

Target Details

Background: Influenza A virus is a major public health threat, killing more than 30,000 people per year in the USA. In early 2009, a novel swine-origin influenza A (H1N1) virus was identified in specimens obtained from patients in Mexico and the United States. The virus spread quickly around the world and on June 11, 2009, the World Health Organization declared it a pandemic. Influenza A virus has one of sixteen possible Hemagglutinin (HA) surface proteins and one of nine possible Neuraminidase (NA) surface proteins. The Hemagglutinin protein facilitates viral attachment while Neuraminidase is involved in viral release. These proteins also elicit immune responses that prevent infection or independently reduce viral replication. The genetic make-up of this swine flu virus is unlike any other: it is an H1N1 strain that combines a triple assortment first identified in 1998 including human, swine, and avian influenza with two new pig H3N2 virus genes from Eurasia, themselves of recent human origin (4). The distinct antigenic properties of the new swine virus compared with seasonal influenza A (H1N1) virus suggest that human immunity against new swine influenza virus is limited, although the age distribution of reported cases suggests some degree of protection in older age groups (5). Antibodies #5235 and 5239 are specific for the seasonal H1N1 influenza Hemagglutinin and will not recognize the corresponding Hemagglutinin sequence from the swine-origin H1N1 influenza (A/California/14/2009 (H1N1)). Similarly, antibodies #5237 and #5241 are specific for the swine-origin H1N1 influenza Hemagglutinin and will not recognize the seasonal H1N1 Hemagglutinin (A/Brisbane/59/2007 (H1N1). The Neuraminidase antibodies #5247 and #5249 are specific for seasonal and swine-origin H1N1 influenza Neuraminidase, respectively.