anti-Rat (Rattus) DDX58 antibody for Western Blotting

Recommended DDX58 Antibody (supplied by: Log in to see )

Antigen
DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
  • RIG-I
  • RIGI
  • RLR-1
  • 6430573D20Rik
  • C330021E21
  • RHIV-1
  • RIG-1
  • DExD/H-box helicase 58
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
  • DEXD/H-box helicase 58
  • DDX58
  • Ddx58
Reactivity
Mouse (Murine), Rat (Rattus), Human
161
72
31
2
2
2
Host
Rabbit
122
32
11
5
Clonality
Polyclonal
Conjugate
This DDX58 antibody is un-conjugated
11
7
5
2
2
2
2
2
2
2
2
2
2
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
124
52
44
29
27
16
15
9
8
7
3
1
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Catalog No. ABIN3023649
$ 393.25
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.8156 ABIN6266336 ELISA IHC WB Rabbit IgG Log in to see Polyclonal 0
10.8156 ABIN2434244 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 0
9.3156 ABIN2476365 IHC (p) WB Rabbit IgG Log in to see Polyclonal 4
7.8156 ABIN5544929 WB Rabbit IgG Log in to see Polyclonal 0
7.8156 ABIN2432609 ELISA WB Rabbit IgG Log in to see Polyclonal 0
7.8156 ABIN1031791 IHC ELISA WB Rabbit Log in to see Polyclonal 0
7 ABIN730663 IF (p) IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
5.5 ABIN1003083 IHC ELISA WB Rabbit IgG Log in to see Polyclonal 4
4.8156 ABIN5518906 WB Rabbit IgG AA 871-925 Log in to see Polyclonal 0
4.8156 ABIN5965558 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5555173 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN730672 IHC (p) WB HRP Rabbit IgG Log in to see Polyclonal 0
1 ABIN730665 IHC (p) WB Biotin Rabbit IgG Log in to see Polyclonal 0
1 ABIN6291996 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN200133 IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5899218 IHC WB Rabbit Log in to see Polyclonal 0
1 ABIN1888240 IHC ELISA WB Rabbit Log in to see Polyclonal 0
1 ABIN5647547 WB Rabbit IgG Log in to see Polyclonal 0

General

Antigen DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) Antibodies
Reactivity Mouse (Murine), Rat (Rattus), Human
(161), (72), (31), (2), (2), (2)
Host Rabbit
(122), (32), (11), (5)
Clonality
Polyclonal
Conjugate This DDX58 antibody is un-conjugated
(11), (7), (5), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(124), (52), (44), (29), (27), (16), (15), (9), (8), (7), (3), (1)
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Product Details anti-DDX58 Antibody

Target Details DDX58 Application Details Handling Images
Purification Affinity purification
Immunogen A synthetic peptide of human DDX58
Isotype IgG
Plasmids, Primers & others

Target Details DDX58

Product Details anti-DDX58 Antibody Application Details Handling Images back to top
Antigen
Alternative Name DDX58 (DDX58 Antibody Abstract)
Background Antiviral innate immunity depends on the combination of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and RNA helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including DNA, single-stranded RNA (ssRNA), double-stranded RNA (dsRNA), and glycoproteins. These antiviral proteins are found in different cell compartments, TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsRNA and two amino-terminal caspase recruitment domains (CARD) that are required for triggering downstream signaling. Rig-I binds both dsRNA and viral ssRNA that contains a 5-triphosphate end not seen in host RNA. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses. The CARD domain of the helicases, which is sufficient to generate signaling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-kappaB, TBK1/IKKepsilon, and IRF-3/IRF-7.
Synonyms: DDX58, DKFZp434J1111, DKFZp686N19181, FLJ13599, RIG-I
Gene ID 23586
UniProt O95786
Pathways Activation of Innate immune Response, Hepatitis C

Application Details

Product Details anti-DDX58 Antibody Target Details DDX58 Handling Images back to top
Application Notes Recommended dilutions: WB: 1:500-1:2000, IHC: 1:50-1:200
Restrictions For Research Use only

Handling

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Images back to top
Format Liquid
Buffer PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze / thaw cycles
Storage -20 °C/-80 °C

Images

Product Details anti-DDX58 Antibody Target Details DDX58 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Western Blotting (WB) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Western blot analysis of extracts of various cell lines, using DDX58 antibody.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Immunohistochemistry of paraffin-embedded human prostate using DDX58 antibody.
Immunofluorescence (IF) image for anti-DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 58 (DDX58) antibody (ABIN3023649) Immunofluorescence analysis of HeLa cells using DDX58 antibody.