GFP antibody (Biotin)

Catalog No. ABIN100087

Supplier Product No.: 600-106-215

This Goat Polyclonal antibody specifically detects GFP in WB, ELISA and IHC. It exhibits reactivity toward Aequorea victoria and has been mentioned in 15+ publications.

Quick Overview for GFP antibody (Biotin) (ABIN100087)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-GFP Antibody (Biotin)

Supplier Product No.: 600-106-215

Supplier: Rockland

Purpose: GFP (GOAT) Antibody Biotin Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum.

Characteristics: Synonyms: goat anti-GFP Antibody biotin Conjugation, biotin conjugated goat anti-GFP antibody, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Purification: Anti-GFP was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Anti-GFP Biotin Conjugated Antibody has been tested by ELISA and western blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.

Western Blot Dilution: 1:2,000 - 1:10,000

ELISA Dilution: 1:50,000 - 1:80,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 1.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-GFP at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-GFP antibody (Biotin) (ABIN100087)

Jongsma, Bakker, Cabukusta, Liv, van Elsland, Fermie, Akkermans, Kuijl, van der Zanden, Janssen, Hoogzaad, van der Kant, Wijdeven, Klumperman, Berlin, Neefjes: "SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport." in: The EMBO journal, Vol. 39, Issue 6, pp. e102301, (2020) (PubMed).

Zöller, Schneider, Kleimeyer, Masuda, Potru, Pfeifer, Blank, Prinz, Spittau: "Silencing of TGFβ signalling in microglia results in impaired homeostasis." in: Nature communications, Vol. 9, Issue 1, pp. 4011, (2019) (PubMed).

Bohlen, El-Nahal, Sommer: "Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques." in: Frontiers in neuroanatomy, Vol. 13, pp. 84, (2019) (PubMed).

Jangphattananont, Sato, Imamura, Sakai, Terakado, Murakami, Barker, Oshima, Oshima, Takagi, Kato, Yano, Matsumoto: "Distinct Localization of Mature HGF from its Precursor Form in Developing and Repairing the Stomach." in: International journal of molecular sciences, Vol. 20, Issue 12, (2019) (PubMed).

Maeder, Kim, Liang, Kaganovsky, Shen, Li, Li, Wang, Xu, Li, Xiang, Ding, Shen: "The THO Complex Coordinates Transcripts for Synapse Development and Dopamine Neuron Survival." in: Cell, Vol. 174, Issue 6, pp. 1436-1449.e20, (2019) (PubMed).

Ge, Kang, Cassidy, Moon, Lewis, Wong, Foster, Craig: "Clptm1 Limits Forward Trafficking of GABAA Receptors to Scale Inhibitory Synaptic Strength." in: Neuron, Vol. 97, Issue 3, pp. 596-610.e8, (2019) (PubMed).

Koerver, Papadopoulos, Liu, Kravic, Rota, Brecht, Veenendaal, Polajnar, Bluemke, Ehrmann, Klumperman, Jäättelä, Behrends, Meyer: "The ubiquitin-conjugating enzyme UBE2QL1 coordinates lysophagy in response to endolysosomal damage." in: EMBO reports, Vol. 20, Issue 10, pp. e48014, (2019) (PubMed).

Yousefi, Günther, Hörner, Chalupsky, Wess, Brandl, Smith, Fleck, Kunkel, Zurbriggen, Höfer, Weber, Schamel: "Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor." in: eLife, Vol. 8, (2019) (PubMed).

Goldmann, Wieghofer, Jordão, Prutek, Hagemeyer, Frenzel, Amann, Staszewski, Kierdorf, Krueger, Locatelli, Hochgerner, Zeiser, Epelman, Geissmann, Priller, Rossi, Bechmann, Kerschensteiner, Linnarsson et al.: "Origin, fate and dynamics of macrophages at central nervous system interfaces. ..." in: Nature immunology, Vol. 17, Issue 7, pp. 797-805, (2017) (PubMed).

Sztal, Zhao, Williams, Oorschot, Parslow, Giousoh, Yuen, Hall, Costin, Ramm, Bird, Busch-Nentwich, Stemple, Currie, Cooper, Laing, Nowak, Bryson-Richardson: "Zebrafish models for nemaline myopathy reveal a spectrum of nemaline bodies contributing to reduced muscle function." in: Acta neuropathologica, Vol. 130, Issue 3, pp. 389-406, (2016) (PubMed).

De Henau, Tilleman, Vangheel, Luyckx, Trashin, Pauwels, Germani, Vlaeminck, Vanfleteren, Bert, Pesce, Nardini, Bolognesi, De Wael, Moens, Dewilde, Braeckman: "A redox signalling globin is essential for reproduction in Caenorhabditis elegans." in: Nature communications, Vol. 6, pp. 8782, (2016) (PubMed).

Baek, Copeland, Yun, Kwon, Guemez-Gamboa, Schaffer, Kim, Kang, Song, Mathern, Gleeson: "An AKT3-FOXG1-reelin network underlies defective migration in human focal malformations of cortical development." in: Nature medicine, Vol. 21, Issue 12, pp. 1445-54, (2016) (PubMed).

Kang, Ge, Cassidy, Lam, Luo, Moon, Lewis, Molday, Wong, Foster, Craig: "A combined transgenic proteomic analysis and regulated trafficking of neuroligin-2." in: The Journal of biological chemistry, Vol. 289, Issue 42, pp. 29350-64, (2014) (PubMed).

Panter, Jain, Leonhardt, Ha, Cresswell: "Dynamics of major histocompatibility complex class I association with the human peptide-loading complex." in: The Journal of biological chemistry, Vol. 287, Issue 37, pp. 31172-84, (2012) (PubMed).

Jain, Liu, Xiang, Ha: "Single-molecule pull-down for studying protein interactions." in: Nature protocols, Vol. 7, Issue 3, pp. 445-52, (2012) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Background: Conjugated Anti-GFP is ideal for western blotting, ELISA and Immunohistochemistry. Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.

UniProt: P42212