Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (HRP)

Details for Product No. ABIN101955, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
4679
2535
1881
1066
1005
860
300
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Rabbit
2848
2736
2291
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
125
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
2
2
1
1
1
1
1
Host
Donkey
6701
4830
1795
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality
Polyclonal
Conjugate
HRP
2235
1871
1740
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
6
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Dot Blot (DB), Immunoelectron Microscopy (IEM), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Immunogen Anti-Rabbit IgG was produced by repeated immunization with rabbit whole IgG molecule in donkey.
Isotype IgG
Specificity IgG (H&L)
Characteristics Anti-Rabbit IgG peroxidase conjugated antibody generated in donkey detects specifically rabbit IgG. This secondary peroxidase conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
Concentration Definition: by UV absorbance at 280 nm
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Donkey Serum, Rabbit IgG and Rabbit Serum.
Background Synonyms: Rabbit Antibody peroxidase conjugated, Dk-a-Rabbit peroxidase conjugated IgG, Rabbit proxidase conjugated Antibody in donkey, Rabbit Secondary peroxidase conjugated Antibody, peroxidase.
Research Area Immunology, Secondary Antibodies
Application Notes Anti-Rabbit IgG peroxidase conjugated antibody is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
Restrictions For Research Use only
Format Lyophilized
Reconstitution Restore with deionized water (or equivalent)
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Gentamicin sulfate
Handling Advice Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Supplier Images
Western Blotting (WB) image for Donkey anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) (ABIN101955) Western Blot of Peroxidase conjugated Donkey anti-Rabbit IgG antibody. Lane 1: Rabbit...
Background publications Farr, Nakane: "Immunohistochemistry with enzyme labeled antibodies: a brief review." in: Journal of immunological methods, Vol. 47, Issue 2, pp. 129-44, 1982 (PubMed).