Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) Antibody

Details for Product No. ABIN102136, Supplier: Log in to see
Antigen
Epitope
Heavy & Light Chain
6012
1904
1869
1159
274
267
266
124
33
30
30
15
12
12
12
12
11
7
3
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
2740
2674
2192
1494
1370
700
664
447
434
362
359
354
275
258
257
147
115
84
43
26
24
21
12
12
8
7
5
5
4
4
4
3
3
3
2
2
2
2
2
2
1
1
1
Host
Goat
6468
4816
1755
887
458
274
87
54
43
35
22
8
6
5
4
1
1
1
Clonality
Polyclonal
Application
ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
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Immunogen

Immunogen: Rat IgG whole molecule

Isotype IgG
Specificity IgG (H&L)
Cross-Reactivity Rat (Rattus)
Cross-Reactivity (Details) Pre-Adsorbed
Characteristics Concentration Definition: by UV absorbance at 280 nm
Purification Anti-RAT IgG (H&L) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgG and Rat Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Sheep Serum Proteins.
Sterility Sterile filtered
Antigen
Background

Synonyms: Goat anti-Rat, Rat IgG Secondary Antibody, Rat Ab in Goat

Background: RAT IgG (H&L) (GOAT) Antibody was generated in goat detects specifically Rat IgG whole molecule. Anti-Rat IgG antibosy is ideal for investigators involved in serum protein component research.

Application Notes

Immunohistochemistry Dilution: 1:500 - 1:2,000

Application Note: Anti-Rat IgG antibody is suitable for ELISA, western blot, and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. Specific conditions should be optimized by user.

ELISA Dilution: 1:10,000 - 1:40,000

Western Blot Dilution: 1:1,000 - 1:4,000

Restrictions For Research Use only
Format Liquid
Concentration 1.1 mg/mL
Buffer

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (ABIN102136) Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (Min X Bv Ch Gt GP Ham Hs Hu Ms Rb...
Western Blotting (WB) image for Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (ABIN102136) Goat anti-Rat (Rattus) IgG (Heavy & Light Chain) antibody (Image 2)