Rabbit anti-Turkey IgG (Heavy & Light Chain) Antibody (HRP)
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- Target See all IgG products
- IgG
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Binding Specificity
- Heavy & Light Chain
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Reactivity
- Turkey
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- HRP
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Application
- ELISA
- Specificity
- IgG (H&L)
- Characteristics
- Concentration Definition: by UV absorbance at 280 nm
- Purification
- This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-HRP, anti-Rabbit Serum, Turkey IgG and Turkey Serum.
- Immunogen
- Turkey IgG whole molecule
- Isotype
- IgG
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- Application Notes
- This product has been assayed against 1.0 µg of Turkey IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4000 to 1:20000 of the reconstitution concentration is suggested for this product.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Restore with deionized water (or equivalent)
- Concentration
- 10.0 mg/mL
- Buffer
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Preservative
- Gentamicin sulfate
- Handling Advice
- Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
- Storage
- 4 °C
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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