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Histone H4 antibody (meLys20)

Reactivity: Human WB, IF, ELISA, ChIP, DB, ICC, IHC (p), FACS Host: Mouse Monoclonal 5E10-D8 unconjugated
Catalog No. ABIN1042605
  • Target See all Histone H4 Antibodies
    Histone H4
    Binding Specificity
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    • 1
    • 1
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    meLys20
    Reactivity
    • 324
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    • 256
    • 25
    • 14
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    • 12
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    • 7
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    Human
    Host
    • 362
    • 22
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    Mouse
    Clonality
    • 337
    • 48
    • 1
    Monoclonal
    Conjugate
    • 215
    • 21
    • 15
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    This Histone H4 antibody is un-conjugated
    Application
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    Western Blotting (WB), Immunofluorescence (IF), ELISA, Chromatin Immunoprecipitation (ChIP), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)
    Specificity
    Specific for K20 mono-methylated histone H4, no cross-reactivity with K20 tri-, di-, or unmethylated histone H4, no cross-reactivity with histone H3.
    Cross-Reactivity
    Arabidopsis thaliana, Drosophila melanogaster, Mammalian, Xenopus laevis
    Cross-Reactivity (Details)
    Human. Predicted to work with: Xenopus laevis, Arabidopsis thaliana, Fruit fly (Drosophila melanogaster), all Mammals
    Purification
    Protein G
    Immunogen
    Synthetic peptide within Human Histone H4 (mono methyl K20)(Immunogen sequence: 15-AKRHR(Kme)VLRDN-25 (branched))
    Clone
    5E10-D8
    Isotype
    IgG1
    Top Product
    Discover our top product Histone H4 Primary Antibody
  • Application Notes
    Optimal antibody dilution should be determined by titration, however as a guideline try, WB: Use a concentration of 1 μg/mL. Detects a band of approximately 13 kDa (predicted molecular weight: 11 kDa).Can be blocked with Histone H4 peptide - mono methyl K20 
    Comment

    Myeloma, fusion partners: X63-Ag8.653

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Purified antibody (from supernatant) containing PBS + 0.1 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
  • Target
    Histone H4
    Abstract
    Histone H4 Products
    Synonyms
    hist1h4l antibody, H1ft antibody, Hist4 antibody, Hist4h4 antibody, H4 antibody, X04652 antibody, FO108 antibody, H4/n antibody, H4F2 antibody, H4FN antibody, HIST2H4 antibody, H4C13 antibody, H4C7 antibody, F8N16.2 antibody, F8N16_2 antibody, histone H4 antibody, BcDNA:RH52884 antibody, CG3379 antibody, Dmel\CG3379 antibody, H4r antibody, His4-88CD antibody, His4R antibody, histone cluster 1, H4a L homeolog antibody, histone cluster 1 H4 family member B antibody, histone cluster 2, H4 antibody, histone cluster 2 H4 family member a antibody, histone H4 antibody, Histone H4 replacement antibody, Histone H4 antibody, hist1h4a.L antibody, Hist1h4b antibody, Hist2h4 antibody, HIST2H4A antibody, H4 antibody, H4C14 antibody, HIS4 antibody, HIST2H4 antibody, His4r antibody, his-38 antibody
    Background
    Synonyms:
    H4F2 antibody,H4FN antibody,HIST1H4 antibody,HIST2H4 antibody,HISTH4H4 antibody,methyl histone H4 antibody,methylated histone H4 antibody
    Gene ID
    318846, 3771854, 3771893
    OMIM
    142750
    UniProt
    P59259, P84040, P02304, P62805, P62799
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