SLC12A4 antibody (AA 932-1043)

Catalog No. ABIN1043014

Product Details anti-SLC12A4 Antibody

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Binding Specificity: AA 932-1043

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This SLC12A4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (fixed cells) (IF/ICC), Immunoprecipitation (IP)

Purpose: Anti-K+/Cl-Cotransporter (KCC2) Mouse Monoclonal Antibody

Specificity: This antibody recognizes human, mouse and rat KCC2.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein G affinity chromatography.

Immunogen: Fusion protein consisting of aa 932-1043 of rat KCC2 (accession no. NP_599190).

Clone: S1-12

Isotype: IgG2a

Application Details

Application Notes: Immunoblotting: use at 3 μg/mL. A band of ~140 kDa is detected.
Immunohistochemistry: use at 1-5 μg/mL.
Positive control: Adult rat brain
These are recommended concentrations,
Enduser should determine optimal concentrations for their applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 50 % glycerol, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: This antibody is stable for at least one (1) year at -20°C.

Target Details for SLC12A4

Target: SLC12A4 (Solute Carrier Family 12 (Potassium-Chloride Transporter) Member 4 (SLC12A4))

Alternative Name: K+/Cl- Cotransporter (SLC12A4 Products)

Synonyms: KCC1 antibody, kcc1 antibody, SLC12A4 antibody, AW546649 antibody, RBCKCC1 antibody, Kcc1 antibody, solute carrier family 12 member 4 antibody, solute carrier family 12 (potassium/chloride transporter), member 4 L homeolog antibody, solute carrier family 12, member 4 antibody, SLC12A4 antibody, slc12a4.L antibody, Slc12a4 antibody

Background: Solute carrier family 12 member 5,Mediates electroneutral potassium-chloride cotransport in mature neurons and is required for neuronal Cl(-) homeostasis. As major extruder of intracellular chloride, it establishes the low neuronal Cl(-) levels required for chloride influx after binding of GABA-A and glycine to their receptors, with subsequent hyperpolarization and neuronal inhibition (PubMed:9930699). Involved in the regulation of dendritic spine formation and maturation (PubMed:22345354). {PubMed:22345354, PubMed:9930699}.,KCC2 is a member of the cation-chloride cotransporter gene family. KCCs lower intracellular chloride concentrations below the electrochemical equilibrium potential. Depending on the concentration gradients of potassium and chloride, KCC2 can operate as a net efflux or influx pathway. KCC2 is expressed at high levels in neurons throughout the nervous system and is localized at inhibitory synapses of the spinal cord. Studies in mice have shown that KCC2 reduces GABA's inhibitory signaling resulting in motor defects, epilepsy, and anxiety-like behavior.,Membrane, Multi-pass membrane protein.,Electroneutral potassium-chloride cotransporter 2, Furosemide-sensitive K-Cl cotransporter, K-Cl cotransporter 2, rKCC2, Neuronal K-Cl cotransporter

NCBI Accession: NP_599190

UniProt: Q63633