Rabbit anti-Mouse (Murine) IgG (Chain gamma) Antibody (Atto 550)

Details for Product No. ABIN1043989, Supplier: Log in to see
Antigen
Epitope
Chain gamma
6236
2031
1776
1135
782
648
372
304
34
34
31
15
12
12
12
12
11
7
3
3
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
3081
2913
2381
1662
1509
786
737
489
476
411
401
395
342
298
271
160
159
111
55
34
30
26
16
15
12
10
8
7
5
5
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Host
Rabbit
7134
5285
1973
1077
507
362
88
84
51
35
23
15
8
5
3
1
1
Clonality
Polyclonal
Conjugate
Atto 550
2227
1823
1751
1266
794
718
430
343
235
147
145
140
140
116
113
112
102
98
94
93
92
91
89
84
81
80
78
75
73
72
68
68
65
61
55
54
54
54
54
53
53
53
53
53
53
52
48
43
41
37
32
31
23
23
22
20
19
18
17
16
15
15
15
14
14
14
12
12
12
12
12
11
11
10
10
10
10
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
6
6
6
5
5
5
5
5
5
5
5
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunoassay (IA)
Options
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Immunogen highly purified mouse IgG gamma 1, gamma 2a, gamma 2b and gamma 3 proteins
Isotype IgG
Characteristics Anti-Mouse IgG (gamma 1, 2a, 2b and 3 chain) conjugated to ATTO 550 is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
This product is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Purification This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. This product shows balanced reactivity to Mouse IgG1, IgG2a, IgG2b and IgG3 proteins and is suitable to screen IgG class hybridoma clones. Minimal cross reactivity is observed against other Mouse immunoglobulin classes or light chain proteins.
Antigen
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Restrictions For Research Use only
Format Lyophilized
Reconstitution Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 500 µL
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid cycles of freezing and thawing.
This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Product is photosensitive and should be protected from light.
Storage -20 °C
Storage Comment Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. This product is stable for several weeks at 0 °C as an undiluted liquid.
Expiry Date 12 months
Supplier Images
 image for Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550)
 image for Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (ABIN1043989) Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (Image 2)
Western Blotting (WB) image for Rabbit anti-Mouse (Murine) IgG (Chain gamma) antibody (Atto 550) (ABIN1043989) Western Blot of ATTO 550 Rabbit Anti-Mouse IgG (gamma 1, 2a, 2b, 3) secondary antibod...