Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (TRITC) - Preadsorbed

Catalog No. ABIN1044076

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Golden Syrian Hamster, Armenian Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: TRITC

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum.

Characteristics: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Purification: Preadsorption: Solid phase absorption

Immunogen:

Immunogen: Golden Syrian and Armenian Hamster IgG whole molecules

Isotype: IgG

Labeling Ratio: 2.5

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1000-1:5000

Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

FLISA Dilution: 1:10,000-1:50,000

Flow Cytometry Dilution: 1:500-1:2,500

IF Microscopy Dilution: 1:500-1:2,500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 500 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is photosensitive and should be protected from light.

Storage: RT,4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage, aliquot contents and freeze at -24 °C or below.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Goat F(ab')2 Anti-Hamster IgG Antibody Rhodamine Conjugation, Goat F(ab')2 Anti-Hamster IgG Rhodamine Conjugated Antibody, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Rhodamine Conjugation, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Rhodamine Conjugated Antibody

Background: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Rhodamine Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.