Goat anti-Armenian Hamster IgG (Heavy & Light Chain) Antibody (FITC) - Preadsorbed
-
- Target See all IgG products
- IgG
- Fragment
- F(ab')2 fragment
-
Binding Specificity
- Heavy & Light Chain
-
Reactivity
- Golden Syrian Hamster, Armenian Hamster
-
Host
- Goat
-
Clonality
- Polyclonal
-
Conjugate
- FITC
-
Application
- FLISA, Fluorescence Microscopy (FM)
- Specificity
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Golden Syrian and Armenian Hamster IgG and Golden Syrian and Armenian Hamster Serum.
- Characteristics
- This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
- Purification
- Preadsorption: Solid phase absorption
- Immunogen
-
Immunogen: Golden Syrian and Armenian Hamster IgG whole molecules
- Isotype
- IgG
- Labeling Ratio
- 3.9
-
-
- Application Notes
-
Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
FLISA Dilution: 1:10,000-1:50,000
IF Microscopy Dilution: 1:500-1:2500
- Comment
-
Excitation/Emission wavelength: 494 nm/514 nm
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 500 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
Product is photosensitive and should be protected from light.
Avoid cycles of freezing and thawing. - Storage
- RT,4 °C,-20 °C
- Storage Comment
- Store vial at 4 °C prior to opening. This product is stable at 4 °C as an undiluted liquid. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -24 °C or below.
- Expiry Date
- 12 months
-
- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
-
Synonyms: Goat F(ab')2 Anti-Hamster IgG Antibody Fluorescein Conjugation, Goat F(ab')2 Anti-Hamster IgG FITC Conjugated Antibody, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Antibody Fluorescein Conjugation, Goat F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG FITC Conjugated Antibody
Background: F(ab')2 Anti-Golden Syrian & Armenian Hamster IgG Fluorescein Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
-