Serotonin Receptor 5A antibody (Cytoplasmic Domain)

Catalog No. ABIN1048277

Product Details anti-Serotonin Receptor 5A Antibody

Target: Serotonin Receptor 5A (HTR5A)

Binding Specificity: Cytoplasmic Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 5A antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC)

Brand: IHC-plus™

Specificity: Human 5HT5A Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human (100%) Gorilla, Monkey, Mouse, Rat (94%) Gibbon, Bat, Dog, Elephant, Horse, Turkey, Chicken, Platypus, Lizard, Seabass, Pufferfish, Stickleback (89%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 18 amino acid peptide from 3rd cytoplasmic domain of human 5HT5A Receptor. Percent identity with other species by BLAST analysis: Human (100%), Gorilla, Monkey, Mouse, Rat (94%), Gibbon, Bat, Dog, Elephant, Horse, Turkey, Chicken, Platypus, Lizard, Seabass, Pufferfish, Stickleback (89%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (8 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for Serotonin Receptor 5A

Target: Serotonin Receptor 5A (HTR5A)

Alternative Name: HTR5A / 5-HT5A Receptor (HTR5A Products)

Synonyms: HTR5A antibody, si:dkey-265a7.5 antibody, Htr5 antibody, 5-HT5A antibody, 5HT5A antibody, MR22 antibody, 5-hydroxytryptamine receptor 5A antibody, 5-hydroxytryptamine (serotonin) receptor 5A, genome duplicate a antibody, 5-hydroxytryptamine (serotonin) receptor 5A antibody, HTR5A antibody, htr5aa antibody, Htr5a antibody

Background: Name/Gene ID: HTR5A
Subfamily: Serotonin
Family: GPCR

Synonyms: HTR5A, 5-HT-5, 5-HT-5A, 5-HT5A, 5-HT5a receptor, 5-hydroxytryptamine5a, Serotonin 5-HT5a receptor, Serotonin 5a receptor, Serotonin receptor 5A

Gene ID: 3361

Pathways: JAK-STAT Signaling