DUSP26 antibody (Dual Specificity Phosphatase 26 (Putative)) (C-Term)

Details for Product anti-DUSP26 Antibody No. ABIN1048527
Antigen
  • DUSP24
  • LDP-4
  • MKP8
  • NATA1
  • SKRP3
  • 2310043K02Rik
  • Skrp3
  • RGD1310090
  • dual specificity phosphatase 26
  • dual specificity phosphatase 26 (putative)
  • DUSP26
  • Dusp26
Epitope
C-Term
13
10
4
2
2
1
1
1
1
Reactivity
Bat, Cow (Bovine), Hamster, Horse (Equine), Human, Monkey, Pig (Porcine), Rabbit, Rat (Rattus)
74
31
24
5
5
3
3
3
3
2
2
2
1
1
Host
Rabbit
72
1
Clonality
Polyclonal
Conjugate
This DUSP26 antibody is un-conjugated
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
22
22
20
18
13
6
6
4
2
2
1
Options
Brand IHC-plus™
Immunogen Synthetic 18 amino acid peptide from near C-terminus of human DUSP26. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Pig (100%), Mouse, Opossum, Turkey, Chicken, Platypus (94%), Stickleback, Pufferfish (89%).

Type of Immunogen: Synthetic peptide
Specificity Human DUSP26. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Predicted Reactivity Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Rat, Hamster, Elephant, Panda, Bat, Bovine, Horse, Rabbit, Pig (100%) Mouse, Opossum, Turkey, Chicken, Platypus (94%) Stickleback, Pufferfish (89%).
Purification Immunoaffinity purified
Plasmids, Primers & others Plasmids, Primers & others DUSP26 products on genomics-online (e.g. as negative or positive controls)
Antigen
Alternative Name MKP-8 / DUSP26 (DUSP26 Antibody Abstract)
Background Name/Gene ID: DUSP26
Subfamily: Dual Specificity MKP
Family: Protein Phosphatase

Synonyms: DUSP26, DSP-4, NATA1, SKRP3, MAP kinase phosphatase 8, MKP-8, DUSP24, LDP-4, LDP4, MKP8
Gene ID 78986
Application Notes Approved: IHC, IHC-P (20 μg/mL), WB
Comment

Target Species of Antibody: Human

Assay Procedure

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions For Research Use only
Format Liquid
Concentration Lot specific
Buffer PBS, less than 0.1 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Dual Specificity Phosphatase 26 (Putative) (DUSP26) (C-Term) antibody (ABIN1048527) Anti-DUSP26 antibody ABIN1048527 IHC staining of human breast. Immunohistochemistry ...
Immunohistochemistry (IHC) image for anti-Dual Specificity Phosphatase 26 (Putative) (DUSP26) (C-Term) antibody (ABIN1048527) Anti-MKP-8 / DUSP26 antibody IHC of human liver. Immunohistochemistry of formalin-fix...
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