NET1 antibody (Internal Region)

Catalog No. ABIN1049095

Product Details anti-NET1 Antibody

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NET1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NET1. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ARHGEF3 (60 % ).

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan (100%) Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Opossum, Platypus (93%) Panda, Dog, Horse, Turkey, Chicken (87%) Pig (80%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 15 amino acid peptide from internal region of human NET1. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan (100%), Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Rabbit, Opossum, Platypus (93%), Panda, Dog, Horse, Turkey, Chicken (87%), Pig (80%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NET1

Target: NET1 (Neuroepithelial Cell Transforming 1 (NET1))

Alternative Name: NET1 (NET1 Products)

Synonyms: arhgef8 antibody, net1a antibody, xNET1 antibody, DKFZp459I0423 antibody, NET1 antibody, ARHGEF8 antibody, NET1A antibody, 0610025H04Rik antibody, 9530071N24Rik antibody, AI604373 antibody, AU015857 antibody, Net1a antibody, mNET1 antibody, wu:fb13g03 antibody, wu:fb25c11 antibody, zgc:92121 antibody, neuroepithelial cell transforming 1 antibody, neuroepithelial cell-transforming gene 1 protein antibody, neuroepithelial cell transforming 1 L homeolog antibody, neuroepithelial cell transforming gene 1 antibody, net1 antibody, EDI_348570 antibody, NET1 antibody, Net1 antibody, net1.L antibody

Background: Name/Gene ID: NET1

Synonyms: NET1, ARHGEF8, NET1A, Proto-oncogene p65 Net1

Gene ID: 10276

Pathways: Neurotrophin Signaling Pathway