NR0B2 antibody (Ligand Binding Domain)

Catalog No. ABIN1049128

Product Details anti-NR0B2 Antibody

Target: NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))

Binding Specificity: Ligand Binding Domain

Reactivity: Human, Mouse, Rabbit, Monkey, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NR0B2 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human NR0B2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%) Rat, Bovine, Bat (94%) Elephant, Panda, Horse, Pig, Opossum (88%) Dog (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from ligand-binding domain of human NR0B2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Hamster, Rabbit (100%), Rat, Bovine, Bat (94%), Elephant, Panda, Horse, Pig, Opossum (88%), Dog (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ICC, IF, IHC, IHC-P (4 μg/mL)

Comment:

Target Species of Antibody: Human

Assay Procedure:

The IHC-pro Immunohistochemistry Protocol

Tissue Preparation
Formalin fixation and embedding in paraffin wax

Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.

Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.

Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for NR0B2

Target: NR0B2 (Nuclear Receptor Subfamily 0, Group B, Member 2 (NR0B2))

Alternative Name: NR0B2 / SHP (NR0B2 Products)

Synonyms: SHP antibody, SHP1 antibody, SHP-1 antibody, Shp1 antibody, Shp antibody, NR0B2-A antibody, Nr0b2 antibody, SHP-A antibody, gb:bc058069 antibody, nuclear receptor subfamily 0 group B member 2 antibody, nuclear receptor subfamily 0, group B, member 2 antibody, nuclear receptor subfamily 0, group B, member 2a antibody, NR0B2 antibody, Nr0b2 antibody, nr0b2a antibody

Background: Name/Gene ID: NR0B2
Subfamily: NR0 Knirps-like
Family: NHR

Synonyms: NR0B2, Short heterodimer partner, SHP, Nuclear receptor SHP, Orphan nuclear receptor SHP, Small heterodimer partner, SHP1

Gene ID: 8431

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway