NR6A1 antibody (Ligand Binding Domain)
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- Target See all NR6A1 Antibodies
- NR6A1 (Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1))
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Binding Specificity
- Ligand Binding Domain
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Reactivity
- Human, Mouse, Rat, Rabbit, Dog, Pig, Cow, Horse, Monkey, Bat, Hamster, Chicken
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NR6A1 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human NR6A1 / GCNF. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus (100%) Stickleback, Pufferfish, Zebrafish (94%) Xenopus (89%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 18 amino acid peptide from ligand-binding domain of human NR6A1 / GCNF. Percent identity with other species by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Pig, Turkey, Chicken, Platypus (100%), Stickleback, Pufferfish, Zebrafish (94%), Xenopus (89%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product NR6A1 Primary Antibody
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- Application Notes
- Approved: IHC, IHC-P (4 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- NR6A1 (Nuclear Receptor Subfamily 6, Group A, Member 1 (NR6A1))
- Alternative Name
- NR6A1 / GCNF (NR6A1 Products)
- Synonyms
- GCNF antibody, GCNF1 antibody, NR61 antibody, RTR antibody, hGCNF antibody, hRTR antibody, 1700113M01Rik antibody, Gcnf antibody, NCNF antibody, gcnf antibody, xEmGCNF antibody, gcnf1 antibody, nr61 antibody, rtr antibody, xGCNF antibody, NR6A1 antibody, GCNF-B antibody, si:ch211-167p9.1 antibody, zC167P9.1 antibody, zgc:114189 antibody, nr6a1 antibody, zfGCNF antibody, nuclear receptor subfamily 6 group A member 1 antibody, nuclear receptor subfamily 6, group A, member 1 antibody, nuclear receptor subfamily 6 group A member 1 S homeolog antibody, nuclear receptor subfamily 6, group A, member 1b antibody, nuclear receptor subfamily 6, group A, member 1a antibody, NR6A1 antibody, Nr6a1 antibody, nr6a1.S antibody, nr6a1 antibody, nr6a1b antibody, nr6a1a antibody
- Background
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Name/Gene ID: NR6A1
Subfamily: NR6 Germ cell nuclear factor-like
Family: NHR
Synonyms: NR6A1, Germ cell nuclear factor, HGCNF, NR61, HRTR, RTR, Ncnf, GCNF, GCNF1 - Gene ID
- 2649
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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