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RXFP2 antibody (Extracellular Domain)

RXFP2 Reactivity: Human, Mouse IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1049307
  • Target See all RXFP2 Antibodies
    RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
    Binding Specificity
    • 14
    • 11
    • 5
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Extracellular Domain
    Reactivity
    • 42
    • 13
    • 8
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Mouse
    Host
    • 44
    Rabbit
    Clonality
    • 44
    Polyclonal
    Conjugate
    • 24
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RXFP2 antibody is un-conjugated
    Application
    • 28
    • 23
    • 6
    • 5
    • 5
    • 2
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human RXFP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except RXFP1 (53 % ).
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Marmoset, Elephant, Panda, Bat, Horse (94%) Monkey, Dog, Pig (88%) Bovine, Rabbit, Platypus, Xenopus (82%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from 2nd extracellular domain of human RXFP2. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset, Elephant, Panda, Bat, Horse (94%), Monkey, Dog, Pig (88%), Bovine, Rabbit, Platypus, Xenopus (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product RXFP2 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (18 - 72 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    RXFP2 (Relaxin/insulin-Like Family Peptide Receptor 2 (RXFP2))
    Alternative Name
    RXFP2 / LGR8 (RXFP2 Products)
    Synonyms
    GPR106 antibody, GREAT antibody, INSL3R antibody, LGR8 antibody, LGR8.1 antibody, RXFPR2 antibody, Gpcr antibody, Lgr8 antibody, Gpr106 antibody, Great antibody, lgr8.1 antibody, rxfp2 antibody, rxfp2b antibody, zgc:63668 antibody, relaxin/insulin like family peptide receptor 2 antibody, relaxin/insulin-like family peptide receptor 2 antibody, relaxin/insulin-like family peptide receptor 2a antibody, RXFP2 antibody, Rxfp2 antibody, rxfp2a antibody
    Background
    Name/Gene ID: RXFP2
    Subfamily: Relaxin
    Family: GPCR

    Synonyms: RXFP2, G-protein coupled receptor 106, GPR106, INSL3R, LGR8, GREAT, Relaxin receptor 2, LGR8.1, RXFPR2
    Gene ID
    122042
    Pathways
    cAMP Metabolic Process, Myometrial Relaxation and Contraction
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