SLC1A5 antibody (C-Term)
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- Target See all SLC1A5 Antibodies
- SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This SLC1A5 antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SLC1A5. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
- Predicted Reactivity
- Percent identity with other species by BLAST analysis: Human (100%) Gibbon, Monkey, Rat (93%) Marmoset, Hamster, Elephant, Horse (87%) Mouse, Bat, Bovine (80%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic 15 amino acid peptide from C-Terminus of human SLC1A5. Percent identity with other species by BLAST analysis: Human (100%), Gibbon, Monkey, Rat (93%), Marmoset, Hamster, Elephant, Horse (87%), Mouse, Bat, Bovine (80%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SLC1A5 Primary Antibody
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- Application Notes
- Approved: IHC, IHC-P (2.5 μg/mL)
- Comment
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Target Species of Antibody: Human
- Assay Procedure
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The IHC-pro Immunohistochemistry Protocol
Tissue Preparation
Formalin fixation and embedding in paraffin wax
Tissue Sectioning
Make 4-µm sections and place on pre-cleaned and charged microscope slides.
Heat in a tissue-drying oven for 45 minutes at 60°C
Deparaffinization
Wash slides in 3 changes of xylene – 5 minutes each at room temperature.
Rehydration
Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
Rinse slides in gentle running distilled water – 5 minutes at room temperature.
Antigen retrieval
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
Remove from heat and let stand at room temperature in buffer - 20 minutes
Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.
Immunostaining
Do not allow tissues to dry at any time during the staining procedure.
Apply a universal protein block – 20 minutes at room temperature.
Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
Rinse slides 1X TBST – 1 minute at room temperature.
Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
Rinse slides in 1X TBST - 1 minute at room temperature.
Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
Wash slides in distilled water – 1 minute at room temperature.
Dehydrate
This method should only be used if the chromogen substrate is alcohol insoluble.
Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
Wash slides in 3 changes of xylene – 1 minute each at room temperature.
Apply coverslip - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, less than 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- SLC1A5 (Solute Carrier Family 1 Member 5 (SLC1A5))
- Alternative Name
- SLC1A5 / ASCT2 (SLC1A5 Products)
- Synonyms
- aaat antibody, asct2 antibody, atbo antibody, m7v1 antibody, m7vs1 antibody, r16 antibody, rdrc antibody, AAAT antibody, ASCT2 antibody, ATBO antibody, M7V1 antibody, M7VS1 antibody, R16 antibody, RDRC antibody, Slc1a7 antibody, Asct2 antibody, H4-ASCT2 antibody, solute carrier family 1 (neutral amino acid transporter), member 5 antibody, solute carrier family 1 member 5 S homeolog antibody, solute carrier family 1 member 5 antibody, slc1a5 antibody, slc1a5.S antibody, SLC1A5 antibody, Slc1a5 antibody
- Background
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Name/Gene ID: SLC1A5
Subfamily: Sodium:dicarboxylate symporter
Family: Transporter
Synonyms: SLC1A5, ATBO, ASCT2, ATB0, Baboon M7 virus receptor, AAAT, M7VS1, M7V1, R16, RDRC, RD114 virus receptor, RDR, ATB(0) - Gene ID
- 6510
- Pathways
- Dicarboxylic Acid Transport, Warburg Effect
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