PCNA antibody (AA 111-122)

Catalog No. ABIN1049498

The Goat Polyclonal anti-PCNA antibody has been validated for WB, IHC and ELISA. It is suitable to detect PCNA in samples from Human, Mouse, Rat and Pig.

Quick Overview for PCNA antibody (AA 111-122) (ABIN1049498)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human, Mouse, Rat, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-PCNA Antibody

Binding Specificity: AA 111-122

Purpose: PCNA (aa111-122)

Sequence: EAPNQEKVSD YE

Specificity: Reported variants represent identical protein: NP_872590.1, NP_002583.1

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EAPNQEKVSDYE, from the internal region of the protein sequence according to NP_002583.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Tonsil. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 30 kDa band observed in lysates of cell line Jurkat (calculated MW of 28.8 kDa according to Human NP_002583.1). Approx.35 kDa band was observed in lysates of cell line NIH3T3 and in Mouse and Rat Testis, and approx 33 kDa in Pig Spleen

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background: PCNA, proliferating cell nuclear antigen, MGC8367, DNA polymerase delta auxiliary protein, OTTHUMP00000030189, OTTHUMP00000030190, cyclin

Gene ID: 5111, 18538, 25737

NCBI Accession: NP_002583

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy