Aprotinin antibody

Catalog No. ABIN1077832

Product Details anti-Aprotinin Antibody

Target: Aprotinin

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aprotinin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Specificity: It has been selected for its ability to recognize AP in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Native aprotinin of humanThe aprotinin is the small protein pancreatic trypsin inhibitor, or BPTI, which inhibits trypsin and related proteolytic enzymes. Aprotinin was used as a medication administered by injection to reduce bleeding during complex surgery, such as heart and liver surgery. In cardiac surgery with a high risk of significant blood loss, aprotinin significantly reduced bleeding, mortality and hospital stay. Beneficial effects were also reported in high-risk orthopedic surgery. In liver transplantation, initial reports of benefit were overshadowed by concerns about toxicity.
The antibody is a rabbit polyclonal antibody raised against AP.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for Aprotinin

Target: Aprotinin

Alternative Name: Aprotinin (AP) (Aprotinin Products)

Synonyms: BPI antibody, BPTI antibody, pancreatic trypsin inhibitor-like antibody, pancreatic trypsin inhibitor antibody, LOC100158011 antibody, PTI antibody