Rabbit anti-Mouse IgE Antibody

Catalog No. ABIN1078164

Product Details

Target: IgE

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: It has been selected for its ability to recognize IgE in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Native Protein IgE Immunoglobulin E (IgE) is a class of antibody that has been found only in mammals. IgE's main function is immunity to parasites such as parasitic worms. IgE also plays an essential role in the immediate hypersensitivity states, such as asthma, hay fever, atopic dermatitis, and other well-known manifestations of atopic patients. IgE is present in normal individuals at very low concentrations, only 0.05 % of the Ig concentration.
The antibody is a rabbit polyclonal antibody raised against IgE.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 µg/mL

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details

Target: IgE

Abstract: IgE Products

Background: Immunoglobulin E (IgE) is a class of antibody that has been found only in mammals. IgE's main function is immunity to parasites such as parasitic worms. IgE also plays an essential role in the immediate hypersensitivity states, such as asthma, hay fever, atopic dermatitis, and other well-known manifestations of atopic patients. IgE is present in normal individuals at very low concentrations, only 0.05 % of the Ig concentration.